ISOLASI DAN IDENTIFIKASI BAKTERI PADA SUSU MASTITIS SUBKLINIS DI KOPERASI TERNAK SARONO MAKMUR SABRANG WETAN WUKIRSARI CANGKRINGAN SLEMAN

EKO WAHYU HERU PRAYITNO, drh. Slamet Raharjo, MP.

2013 | Skripsi | KEDOKTERAN HEWAN

Mastitis subklinis merupakan salah satu penyebab penurunan produksi dan kualitas susu. Berbagai spesies bakteri dapat menjadi penyebab mastitis subklinis. Penelitian ini bertujuan mengisolasi bakteri pada susu mastitis subklinis pada sapi perah di Koperasi Ternak Sarono Makmur Sabrang Wetan Wukirsari Cangkringan Sleman Jogjakarta. Sebanyak 20 sampel susu sapi perah dari Koperasi Ternak Sarono Makmur Sabrang Wetan Wukirsari Cangkringan Sleman digunakan dalam penelitian ini. Sampel susu dilakukan uji mastitis dengan metode California Mastitis Test (CMT) dan diperoleh hasil 16 sampel positif mastitis subklinis dan 4 sampel negatif. Sampel susu yang positif mastitis subklinis kemudian dilakukan isolasi pada beberapa media selektif dan dilakukan pengujian biokimiawi dengan penanaman pada beberapa media seperti gula-gula (manitol, glukosa, laktosa, sukrosa, maltose), uji katalase, uji koagulase, oksidase, uji motilitas, uji simone citrate, uji imvic, uji urease, dan uji DNAse. Hasil isolasi dari 16 sampel yang positif mastitis subklinis berhasil diisolasi bakteri Escherichia coli 11 isolat (68,75%), Bacillus cereus 5 isolat (31,25%), Diplococcus sp 2 isolat (12,5%), Staphylococcus aureus 2 isolat (12,5%), Klebsiella pneumonia 2 isolat (12,5%), Stomacoccus sp 2 isolat (12,5%), Klebsiella ozaena 1 isolat (6,25%), Staphylococcus epidermidis 1 isolat (6,25%) dan Micrococcus sp 1 isolat (6,25%). Persentase Escherichia coli yang tinggi mengindikasikan higienitas pengelolaan sapi perah masih perlu ditingkatkan. Perbaikan sanitasi, pemerahan sesuai prosedur, perbaikan manajemen pemeliharaan sapi diharapkan dapat menekan perkembangan bakteri patogen penyebab mastitis subklinis dalam susu.

Subclinical mastitis was one of causes of decreasing milk production. The various species of bacteria could be the cause of subclinical mastitis. This research was aim to isolate and identify the bacteria in subclinical mastitis milk from Sarono Makmur farm group in Sabrang Wetan Wukirsari Cangkringan Sleman Jogjakarta. Twenty (20) milk samples from Sarono Makmur farm group were use in this research. Milk samples tested with California Mastitis Test (CMT) test for determine the subclinical mastitis and get 16 samples were positive subclinical mastitis and 4 samples were negative. The 16 subclinical mastitis milk samples then do isolation on selective media and biochemical test with planting on some media such as sugars (mannitol, glucose, lactose, sucrose, maltose), catalase test, coagulase test, oxidase, motility test, simone citrate test, IMViC test, urease test, and DNAse test. The results of bacterial isolation from 16 samples subclinical mastitis were found Escherichia coli 11 isolate (68.75%), Bacillus cereus 5 isolate (31.25%), Diplococcus sp 2 isolate (12.5%), Staphylococcus aureus 2 isolate (12.5%), Klebsiella pneumonia, 2 isolate (12.5%), Stomacoccus sp 2 isolate (12.5%), Klebsiella ozaena 1 isolate (6.25%), Staphylococcus epidermidis 1 isolate (6.25%) and Micrococcus sp 1 isolate (6.25%). Escherichia coli found in high percentage (68.75%) indicate the hygiene management of the dairy cow was worse. Improved sanitation, milking procedures, maintenance management, improvement expected to suppress the growth of pathogenic bacteria causing subclinical mastitis in dairy were needed.

Kata Kunci : Mastitis subklinis, Isolasi, Identifikasi, Bakteri