UJI STERILITAS LARYNGOSKOP KAMAR OPERASI GEDUNG BEDAH SENTRAL TERPADU RSUP Dr. SARDJITO

ANTON DJOKO SUYONO, Dr. Muhdar Abubakar, Sp.An(K), KIC.,

2011 | Tesis | S2 Ked.Klinik/MS-PPDS

Latar belakang : Penggunaan blade laryngoscope steril mutlak diperlukan pada tiap pasien, akan tetapi proses dekontaminasi mungkin saja tidak efisien. Blade laryngoscope yang digunakan dalam kamar operasi sangat berpotensi terjadi kontaminasi oleh mikroorganisme patogen melalui transmisi antar pasien. Metode : Desain penelitian ini dengan studi observasional telah dilakukan untuk meneliti sterilitas blade laryngoscope dalam kamar operasi instalasi bedah sentral RSUP. Dr. Sardjito. Ruang lingkup penelitian adalah seluruh blade laryngoscope layak pakai di kamar operasi di GBST RSUP Dr Sardjito Yogyakarta. Pada subyek dilakukan kultur sebelum digunakan pada pasien, dengan penilaian melihat pertumbuhan mikroorganisma yang muncul dengan merujuk kuman komensal pada rongga mulut. Hasil : Identifikasi kultur 67,4% sampel dari blade laryngoscope terdapat pertumbuhan kuman dan 36,2% tidak tumbuh kuman. Identifikasi kuman didapatkan kuman yang bersifat sebagai non patogen 47,8% sampel dan 19,6% sampel kuman pathogen. Flora normal dan kuman pathogen yang ditemukan ini berpotensial menimbulkan terjadinya infeksi nasokomial. Simpulan : Hasil pertumbuhan kuman ditemukan pada 67,4% blade laryngoscope yang terdiri dari kuman non pathogen 47,8% dan kuman pathogen 19,6%.

Background: This reseach is about the use of sterile laryngoscope blade for each patient, the disinfection process of laryngoscope blade probably not efficient. It may be contaminated by pathogen microorganism through transmission between patients. The objective to assess the nature and extent of microbial contamination on the blade of laryngoscopes that were considered to be clean and ready for use in the anaesthetic rooms within the operating of central surgery building Dr Sardjito hospital. Method: This research had already done by using observational study. Specimens were collected from laryngoscope blade which were deemed to be “ready for patient use” in operating theatre room of central surgery building unit Dr. Sardjito hospital. Further identification of all isolates by laryngoscope blade in operating theatre room of central surgery building unit Dr. Sardjito hospital.The main outcome is microorganism pattern on blade laryngoscope which correlate wih normal comensal of mouth. Result : Microbiology culture showed that 67,4% sampel were identified had microorganism growth and 36,2% had not microorganism growth. Microbiology culture showed 47,8% sampel were non pathogen microorganism and 19,6% sampel were pathogen microorganism. Amount of pathogen microorganism and human normal flora are high enough and still have potential for causing direct nasocomial infection to lower respiratoy system. Conclusion : Results of mycrobiologic cultures from laryngoscope blades showed that 67,4% were microorganism grown, 47,8% were non pathogen and 19,6% were pathogen.

Kata Kunci : blade, laryngoscope, dekontaminasi, identifikasi mikroorganisme