The Association of -866G/A Polymorphism in UCP2 Gene and -55C/T Polymorphism in UCP3 Gene with Insulin Resistance in Obese Female Adolescents

DIAN EURIKE S, S. KED, dr. Rina Susilowati, Ph.D.

2011 | Tesis | S2 Ilmu Kedokteran Dasar dan Biomedis

Latar belakang Penelitian yang dilakukan pada tahun 2007 melaporkan bahwa 55,7% dari 79 remaja putri obes di Yogyakarta telah mengalami resistensi insulin. Studi lanjutan dengan 79 remaja putri obes tersebut sebagai subjek penelitian melaporkan bahwa tidak terdapat perbedaan yang bermakna pada pola makan serta aktivitas fisik secara umum antara remaja putri obes yang mengalami resistensi insulin dengan yang tidak mengalami resistensi insulin. Faktor genetik diduga sebagai faktor yang mendasari adanya perbedaan pada remaja putri obes yang mengalami resistensi insulin dengan yang tidak mengalami resistensi insulin. Polimorfisme -866G/A gen UCP2 dan -55C/T gen UCP3 telah dihubungkan dengan terjadinya resistensi insulin pada populasi lain. Tujuan Untuk mengetahui hubungan antara polimorfisme -866G/A gen UCP2 dan -55C/T gen UCP3 dengan resistensi insulin pada remaja putri obes di Yogyakarta. Metode Sebanyak 79 remaja putri obes dilibatkan dalam penelitian ini. Analisis genotipe polimorfisme -866G/A gen UCP2 dan -55C/T gen UCP3 dilakukan dengan metode PCR-RFLP. Hasil Individu homozigot UCP2 -866A/A serta karier alel A memiliki risiko yang lebih tinggi untuk mengalami resistensi insulin (genotipe A/A: OR 2,8; 95% CI 0,7 – 11,6; alel A: OR 1.5; 95% CI 0.8 – 2.8). Peningkatan risiko resistensi insulin juga ditemukan pada individu homozigot UCP3 -55T/T (OR 2.3; 95% CI 0.4 – 14.1) serta karier alel T (OR 1.3; 95% CI 0.7 – 2.5). Kombinasi genotipe dan alel yang berisiko tinggi dari kedua polimorfisme tersebut juga meningkatkan risiko resistensi insulin. Tetapi keseluruhan hasil tersebut tidak bermakna secara statistik (p > 0,05). Kesimpulan Genotipe A/A dan alel A dari polimorfisme UCP2 -866G/A serta genotipe T/T dan alel T dari polimorfisme UCP3 -55C/T meningkatkan risiko resistensi insulin pada remaja putri obes di Yogyakarta, tetapi hasil tersebut tidak bermakna secara statistik.

Background Previous study in 2007 reported that 55.7% of 79 obese female adolescents in Yogyakarta were in insulin resistant state. Further study with those 79 obese female adolescents as the studied population showed there were no significant differences on dietary habits and general physical activity between obese female adolescents who were insulin resistant and those who weren’t. Therefore, it was assumed that genetic factors might underlie the difference between both groups. Genetic factors that have been associated with insulin resistance in other populations are -866G/A polymorphism in UCP2 gene and - 55C/T polymorphism in UCP3 gene. Aim To investigate the association of -866G/A polymorphism in UCP2 gene and - 55C/T polymorphism in UCP3 gene with insulin resistance in obese female adolescents in Yogyakarta. Methods A total of 79 obese female adolescents were included in this crosssectional study. Genotyping of -866G/A polymorphism in UCP2 gene and -55C/T polymorphism in UCP3 gene were performed by PCR-RFLP. Results The UCP2 -866A/A homozygous individuals and UCP2 A allele carriers had higher risk for insulin resistance (A/A genotype: OR 2.8; 95% CI 0.7 – 11.6; A allele: OR 1.5; 95% CI 0.8 – 2.8). Increased risk for insulin resistance was also found in UCP3 -55T/T homozygous individuals (OR 2.3; 95% CI 0.4 – 14.1) and UCP3 -55T carriers (OR 1.3; 95% CI 0.7 – 2.5). The combination of high risk genotypes and alleles from both polymorphisms also increased the risk for insulin resistance. However, all of those results were statistically not significant (p > 0.05). Conclusion This study suggested that although not statistically significant, A/A genotype and A allele of UCP2 -866G/A polymorphism as well as T/T genotype and T allele of UCP3 -55C/T polymorphism increased the risk for insulin resistance in obese female adolescents in Yogyakarta.

Kata Kunci : Polimorfisme -866G/A gen UCP2, polimorfisme -55C/T gen UCP3, resistensi insulin, obesitas, remaja putri.