PENGARUH EPIGALLOCATECHIN GALLATE TERHADAP VIABILITAS DAN PRODUKSI INTERLEUKIN 10 PADA SEL MONONUKLEAR DARAH TEPI PENDERITA PSORIASIS
YUNITA HAPSARI, dr. Agnes Sri Siswati, SpKK(K)
2011 | Tesis | S2 Ked.Klinik/MS-PPDSLatar belakang: Psoriasis merupakan penyakit inflamasi kulit kronik residif diperantarai limfosit T, ditandai dengan hiperproliferasi keratinosit. Insidensi psoriasis meningkat 2 kali lipat selama satu dekade terakhir. Modalitas terapi psoriasis belum efektif untuk mencegah kekambuhan dan menimbulkan efek samping sistemik. EGCG merupakan polifenol utama teh hijau, bersifat anti proliferatif terhadap biakan berbagai sel kanker, berperan penting pada penyakit-penyakit autoimun dan inflamasi sepertipsoriasis sehingga diujicobakan sebagai terapi alternatif psoriasis. Tujuan penelitian: Mengetahui pengaruh pemberian epigallocathecingallate terhadap viabilitas dan produksi IL-10 pada biakan sel mononuklear darah tepi (SMDT) penderita psoriasis. Metode: Sel mononuklear darah tepi diisolasi dari 5 penderita psoriasis dan orang sehat, diinkubasi dalam media RPMI dan EGCG 50, 100, 200 µg/ml selama 48 jam kemudian dilakukan pengukuran viabilitas dan produksi IL-10 dengan metode MTT assay dan IL-10 kits menggunakan ELISA reader. Hasil: EGCG menurunkan viabilitas SMDT penderita psoriasis dan orang sehat secara bermakna pada dosis 100 dan 200 µg/ml. Penurunan viabilitas SMDT pada kontrol sehat melebihi penderita psoriasis secara bermakna pada EGCG dosis 100 µg/ml (p=0.003). Produksi IL-10 penderita psoriasis lebih rendah dibandingkan orang sehat (p=0.012). Ketiga dosis EGCG meningkatkan produksi IL-10 pada kelompok psoriasis (p=0.029) sedangkan pada orang sehat pada dosis 100µg/ml (p=0.001) dan 200 µg/ml (p=0.010). Simpulan: Pada psoriasis terdapat peningkatan jumlah SMDT dan penurunan produksi IL-10 dibanding orang sehat. EGCG menurunkan viabilitas dan meningkatkan produksi IL-10 SMDT penderita psoriasis dan orang sehat.
Introduction: Psoriasis is a chronic recurrent inflammatory skin disease mediated by T cell lymphocytes and characterized with hyperproliferation of keratinocytes. Since there was no effective psoriatic modalities for preventing relapse and have minimal systemic side effects, psoriasis incidence tend to increase twice in the last decade. EGCG is a major green tea polyphenol that have antiproliferative effect on many cancer cells also has a great impact on autoimmune and inflammatory disease marked by abnormality of T cell such as psoriasis. The aim of the study: To know the effect of EGCG on the viability and IL-10 production of peripheral mononuclear blood cells (PBMC) in psoriasis patients. Material and Methods: PBMC isolated from 5 psoriatics and healthy controls incubated for 48 hours in RPMI medium containing of EGCG 50, 100, 200 µg/ml. The viability and IL-10 production were measured using MTT assay and IL-10 kits with ELISA reader. Results: The PBMC viability were decreased by EGCG both in psoriatics and healthy controls significantly at 100 and 200 µg/ml (p<0.05).There was more descent viability of healthy controls PBMC than psoriatic significantly at EGCG 100 µg/ml (p=0.003). There was lower IL-10 production in psoriatic than healthy control (p=0.012). The three doses of EGCG increased IL-10 production on psoriatic (p=0.029) as for healthy control at 100µg/ml (p=0.001) dan 200 µg/ml (p=0.010). Conclusions: There were higher number of PBMC and lower level of IL-10 in psoriatics than in healthy controls. EGCG decreased the viability and increased IL-10 production of PBMCs in both psoriatics and healthy controls.
Kata Kunci : Psoriasis, EGCG,MTT, IL-10, sel mononuklear darah tepi
