Peranan pemberian platelet rich plasma dengan penambahan bovine trombin terhadap kekuatan tarik kulit tikus wistar pada proses penyembuhan pascabedah
HASIBUAN, Siti Rahma, drg. Prihartiningsih, SU.,SpBM(K)
2010 | Tesis |Penyembuhan luka merupakan suatu proses fisiologis yang komplek sebagai respon terhadap adanya luka. Selama proses penyembuhan luka terjadi pembentukan jaringan granulasi, proliferasi epitel, angiogenesis, proliferasi fibroblast, dan deposisi kolagen yang melibatkan berbagai macam hormon pertumbuhan seperti Platelet derived growth faktor (PDGF), transforming growth faktor (TGF) - β dan lain sebagainya.Tujuan penelitian ini adalah untuk mengetahui peranan pemberian platelet rich plasma (PRP) dengan penambahan bovine trombin terhadap kekuatan tarik kulit tikus Wistar pada proses penyembuhan pascabedah hari ke 8. Penelitian ini dilakukan pada 30 ekor tikus Wistar (Rattus Norvegicus) jantan, berat ± 250 gr, umur ± 3 bulan. Tikus dibagi menjadi 3 kelompok secara acak yaitu kelompok A1 (NaCl), kelompok A2 (bovine trombin) dan kelompok A3 (platelet rich plasma dengan penambahan bovine trombin). Tiap kelompok dibuat insisi pada kulit punggung tikus ,sepanjang 3 cm dengan ke dalaman sampai subkutan dan dijahit sebanyak tiga jahitan, kemudian dioleskan 2 kali sehari selama satu minggu. Pada ketiga kelompok didekapitasi pada hari ke 8 lalu dikuliti dan diuji kekuatan tarik kulit. Hasil yang didapat menunjukkan rata-rata kekuatan tarik kulit kelompok A1 (5.61 ± 1.477), A2 (5.64 ± 1.592) dan kelompok A3 (14.79 ± 4.173). Dianalisa dengan uji Krusskal - Wallis dan Mann - Whitney. Hasil analisis statistik dengan uji Krusskal - Wallis pada ketiga kelompok menunjukkan terdapat perbedaan yang bermakna secara statistik pada kekuatan tarik kulit tikus Wistar pascabedah (p<0.05). Hasil uji Mann-Whietney antara kelompok A1 dan A2 tidak terdapat perbedaan yang bermakna secara statistik (p>0.05), sedangkan antara kelompok A1 dan A3, serta antara kelompok A2 dan A3 terdapat perbedaan kekuatan tarik kulit tikus Wistar pascabedah yang sangat bermakna (p < 0.05). Hal ini berarti bahwa kekuatan tarik kulit tikus pascabedah antara tiap kelompok tidak sama. Kesimpulan penelitian ini adalah pemberian platelet rich plasma dengan penambahan bovine trombin dapat meningkatkan kekuatan tarik kulit tikus Wistar pascabedah hari ke 8.
Wound healing is a complex physiological process as a response to wound. During the process, tissues such as granulation, epithelial proliferation, angiogenesis, fibroblast proliferation, and collagen deposition are formed in which various growth hormones like platelet derived growth factor, transforming growth factor - β and others involve. The study aims to find out the roles of platelet rich plasma (PRP) addition with bovine trombin towards the tensile strength of Wistar rat’s skin on the 8th day during post surgery recovery process. The study was conducted to 30 male Wistar rats (Rattus Norvegicus), each of which weighs ± 250 grams, and is 3 months of age. The rats were randomly selected and divided into three groups: Group A1 physiological NaCl solution was applied to the rat’s skin, A2 bovine trombin was applied, and A3 platelet rich plasma addition with bovine trombin was applied, each of which consists of 10 rats. All rats in the three groups were incised 3 cm long with subcutaneous depth on their back, and then were sutured with three stitches, within one week respectively. All rats were decapitated on the 8th day, and then they were scalped, and the tensile strength of their skin was measured. The result suggests that the average tensile strength of skin of group A1 (5.61 + 1.477), A2 (5.64 + 1.592) and group A3 (14.79 + 4.173). The collected data were analyzed with Kruskal Wallis test and Mann-Whietney test. The statistical analysis result with Kruskal Wallis test suggests that there is a significant difference between the tensile strength of Wistar rat’s skin after surgery (p < 0.05). Mann-Whietney test to Group A1 and A2 indicates that there is no significant difference on the tensile strength of Wistar rat’s skin after surgery (p > 0.05), meanwhile the test done to Group A1 and A3, and Group A2 and A3 suggests a significant difference on the tensile strength of Wistar rat’s skin after surgery (p < 0.05). The finding implies that the tensile strength of the rat’s skin among the groups after surgery among varies. The study concludes that Platelet Rich Plasma (PRP) addition with bovine trombin administration can increase the tensile strength of Wistar rat’s skin on the 8th day after surgery.
Kata Kunci : Kekuatan tarik kulit,Pascabedah,NaCL fisiologis,Bovine trombin,Platelet rich plasma (PRP)
