Pengaruh pemberian alpha lipoic acid terhadap sintesis kolagen dan viabilitas sel fibriblas pada biakan fibroblas manusia setelah mendapat pajanan sinar ultra violet A

SARASWATI, Putu Dyah Ayu, dr. Soedirman Sastrodiprojo, Sp.KK(K)

2010 | Tesis | S2 Ilmu Kedokteran Klinik

Penelitian ini bertujuan untuk mengetahui efek Alpha Lipoic Acid (ALA) pada sintesis kolagen dan viabilitas fibroblas setelah mendapat pajanan sinar UltraViolet A (UVA).Biakan fibroblast kulit manusia dipajan sebanyak tiga kali dengan UVA (PAST Sun B- LIPITM) dengan dosis masing masing 3000mJ/cm2. ALA ditambahkan kedalam kultur segera setelah pajanan UVA dan diinkubasikan selama 48 jam. Kadar kolagen terdegradasi, sintesis kolagen diukur dengan sirius binding assay sedangkan viabilitas sel diukur dengan MTT-assay. Data dianalisa dengan menggunakan uji t berpasangan.Hasil penelitian menunjukkan Pajanan UVA menginduksi kolagen degradasi,menghambat síntesis kolagen dan mempengaruhi viabilitas sel. Pemberian ALA setelah pajanan UVA menujukka bahwa ALA tidak mampu meningkatkan síntesis kolagen, viabilitas fibroblas dan menghambat degradasi kolagen. Pemberian ALA tidak memberikan efek terhadap sel jika diberikan setelah pajanan UVA

The aim of the study is to determine the effect of Alpha Lipoic Acid (ALA) on collagen synthesis and cellular viability among UVA irradiated human fibroblasts. Cultured of human fibroblasts were irradiated three times with UVA (PAST Sun B-LIPITM) each of 3000mJ/cm2. ALA was added to the culture following UVA irradiation and incubated for 48 hours. The expression of collagen degradation,collagen synthesis was determined by sirius red binding assay while cell viability was determined by MTT-assay. Data was analysed using paired-t test.UVA induced collagen degradation, inhibit collagen synthesis and affect cellular viability. Supplementation of ALA among UVA irradiated showed that ALA was not sufficient to enhance viability of fibroblasts, to increases collagen synthesis and to inhibit collagen degradation. ALA has no impact when it is supplemented among UVA irradiated human fibroblasts.

Kata Kunci : Alpha lipoic acid,Fibroblas,Sinar UVA