Pengaruh vitamin C terhadap proliferasi dan ekspresi vascular endothelial growth factor pada fibroblas normal

MUNIRA, dr. Sunardi Radiono, SpKK(K)

2009 | Tesis | S2 Ilmu Kesehatan Kulit dan Kelamin

Penelitian ini bertujuan untuk menentukam apakah vitamin C dapat meningkatkan proliferasi fibroblas dan meningkatkan ekspresi VEGF fibroblas normal. Rancangan penelitian yang digunakan adalah eksperimental sederhana, memakai kultur fibroblas kulit normal 1 orang relawan subkultur passage 3. Kultur fibroblas dibagi menjadi 6 keiompok, masing-masing kelompok perlakuan mendapat vitamin C 50 myug/ml, 100 myug/ml, 150 myug/ml, 200 myug/ml 300 myug/rnl dan kelompok tanpa perlakuan sebagai control. Selanjumya dilakukan pengukuran proliferasi fibroblas dengan spektrofotometer menggunakan MTT dan ekspresi VEGF fibroblas muggumkan ELISA. Perbedaan rerata proliferasi dan ekspresi VEGF fibroblast diuji dengan analisis varian satu jalan. Vitamin C terbukti dapat meningkatkan proliferasi fibroblas, namun tidak mampu meningkatkan ekspresi VEGF fibroblas normal.

The aim of this study is to determine whether vitamin C can be able to increase fibroblasts proliferation and increase VEGF expression by normal fibroblasts. A simple experimental study was conducted on 3rd passage normal human skin fibroblasts culture, isolated from one patient. Fibroblast cell culture was divided into 6 groups, each of treated group was given with vitamin C 50 myug/ml, 100 myug/ml, 150 myug/ml, 200 myug/ml, 300 myug/ml and untreated group as a control. Subsequently fibroblast proliferation was measured with spectrophotometer by using MTT-assay and VEGF expression by fibroblasts using ELISA. The mean differences of proliferation and VEGF expression by fibroblasts were analyzed using one-way Anova. Vitamin C is able to increase fibroblasts proliferation, however vitamin C can not increase VEGF expression by normal fibroblasts.

Kata Kunci : Vitamin C,asam askorbat,Proliferasi fibroblas,Ekspresi VEGF fibroblas ; Vitamin C, ascorbic acid, fibroblast proliferation, VEGF expression by fibroblast