Pengaruh aras kuning telur dan jenis agen kryoprotektan dalam pengencer tris terhadap kualitas dan fertilitas spermatozoa domba lokal

ASTUTI SW., Sulvia Dwi, Prof. Ir. Ismaya, M.Sc., Ph.D

2009 | Tesis | S2 Ilmu Peternakan

Penefitian ini benujuan untuk mengetahui pengaruh aras kuning telur, jenis dan aras agen kryoprotektan Serta interaksinya tehadap kualitas spermatozoa, yang meliputi: motilitas, viabilitas , normalitas morfologi serfa fertilitas Spennatozoa domba lokal sebelum pembekuan dan setelah thawing. Sperma ditampung dua kali seminggu selama sebulan, dari delapan ekor domba Iokal menggunakanvagina buatan.Penilaian kualitas sperma segar meliputi volume, warna, bau, konsistenti, pH, motilitas, konsentrasi, viabilitas dan abnormalitas spermatozoa. Sperma diencerkan menggunakan tris-ctric acid-fructose kuning telur dengan empat macam perlakuan aras kuning telur(KT) yaitu 5%,, 10%, 15% dan 20% serta tiga macam perlakuan jenis agenkryprotektan, yaitu gliserol (GLY), ethylene glycol (EG), dan t I’o Wile, @c. Dimethyl sulfoxde (DMSO) masing-masing dengan dua macam perlakuan aras yaitu : 3.2% dan 6,4%. Sperma diekuilibrasi selama empat jam kemudian dikemas dalam midistraw (0,5 m!) menggunakan mesin filling-sealing dan dibekukan serta disimpan dalam nitogen cair (196° C) selama sebulan. Data sperma segar dianalisis menggunakan estimasi mean dan standar deviasi, sedangkan data kualitas spermatozoa sebelum pembekuan dan setelah thawing dianalisis menggunakan analisis variansi dalam Rancangan Acak Lengkap Pola Faktorial 4x3x2. Diperoleh kombinasi perlakuan (KP) terbaik, yaitu : 5% KT; 3,2% GLY (KP) dan 10% KT, 3,2% DMSO (KP II), yang selanyutnya digunakan untuk insenminasi via cervix terhadap 12 ekor betina resipen, dengan dua macam fase sperma yaitu cair (FS I) dan setelah thawing (FS II) Pemeriksaan kebuntingan (PKB) dilakukan dengan metode biopsy vagina dan ultrasonografi (USG). Data dianalisis menggunakan analisis variansi dalam rancangan Acak Lengkap Pola Faktoral 2x2x2x. Angka fertilitas yang tinggi ditunjukan oleh KPJ pada FS II yaitu 83,34% sehingga dapat disimpulkan bahwa 5% KT dan 3,2% GLY merupakan kombinasi perlakuan yang optimum untuk sprema domba lokal karena menunjukan angka fertilitas setelah thawing yang tinggi.

This research was examined the efiect of egg yolk level, variety and level of cryoprotectant agents (CPAs) on the quality of spermatozoa, such as: motility, viability, abnonnaflty of morphology and fertility of local ram spermatozoa, before freezing and after thawing. Sperm was collected twice weekly for a month from eight local ram using artificial vagina and evaluated for fresh sperm quality, such as: volume, colour, smell, consistence. pH, motility, concentration, viability and abnonnallty of morphology of spermatozoa. Sperm was diluted used tris-citric acid- fructoseegg yolk with four treatments of egg yolk level (KT), i. e.'. 5%, 10%, 15%. and 20%. three treatments of variety of (PM, i- 63.: glycerol {GLY}, ethylene glycol (EG), and dimethyl sulfoxide (DMSO), with two treatments of CPAs level, i, e.: 3.2% and 6.4%. Sperm was equilibrated for four hours then was packaging in midistraw {0.5 ml) by fillingssealing machine and cryopreserved in liquid nitrogen {-1 96°C} for a month. Data of fresh semen quality were analyzed by estimation of mean and standar deviation, While the quality of spermatozoa before freezing and after thawing were analyzed by variance analysis in Completely Randomized Design with 4x3x2 factorial design. Two best treatments combination were found, i. e. 1 5% KT; 32% GLY (KP l) and 16% KT; 3.2% DMSO (KP ll), then were used to inseminated 12 ewes by cervical insemination method on two phase of sperm: liquid (PS I) and alter thawing (F3 II). Biopsy vagina and ultrasonography (USG) were used to detected of pregnancy- Data were analyzed with variance analysis in Completely Randomized Design with 2x2x2 factorial design. The best fertility rate was showed by KP l on F8 it, i. e. : 83.34%, so it was concluded that 5% KT and 3.2% GLY was the optimum combination for cryopreservation of local ram Sperm because showed a highly fertility rate after thawing

Kata Kunci : Domba lokal,Spermatozoa,Kuning telur,Agen kryoprotektan,Fertilitas spermatozoa ; Local ram, Spermatozoa, Egg yolk, Cryoprotectant agent,Quality and fertility of spermatozoa