Pengendalian bakteri anggota genus vibrio dengan pemberian ekstrak mangrove

WALUYANINGRUM, Esti, Langkah Sembiring, M.Sc., Ph.D

2009 | Tesis | S2 Biologi

Dalam upaya mengetahui kemampuan ekstrak mangrove untuk mengendalikan aktivitas bakteri Vibrio dan mengetahui golongan senyawa dilakukan seleksi ekstrak mangrove. Sampel mangrove sebanyak 43 berasal dari daerah Cilacap, Situbondo, dan Batam diseleksi berdasarkan aktivitas dan spektrum terhadap 19 anggota genus Vibrio patogen dengan menggunakan metode paper disc diffusion pada double layer agar. Isolasi senyawa anti-Vibrio dilakukan dengan fraksinasi menggunakan partisi cair, kromatografi kolom dan kromatografi lapis tipis. Senyawa anti-Vibrio diidentifikasi menggunakan penampak bercak. Pengujian aktivitas anti- Vibrio dilakukan secara in vitro. Berdasarkan aktivitas dan spektrum antagonisitas didapatkan 2 sampel terpilih 5C dan KBa. Fraksi aktif anti-Vibrio (5C1-3, KBa1-3) berhasil diisolasi. Fraksi aktif 5C da KBa menunjukkan reaksi positif terhadap pereaksi penampak bercak, yang diduga merupakan golongan senyawa alkaloid dan flavonoid. Fraksi aktif 5C1 da KBa1 bersifat bakterisidal pada dosis 3,1 dan 1,56 mg/ml terhadap bakteri V. alginolyticus 26S dan V. Parahaemolyticus 29S. Berdasarkan identifikasi menunjukkan sampel daun R.mucronata dan kulit kayu X. rumphii mempunyai golongan senyawa yang aktif terhadap bakteri Vibrio.

In the effort to know the ability of the extracted mangrove to control the Vibrio bacteria’s activities and to find out the related compounds which exist on the mangrove extract. There were 43 samples mangrove extract from Cilacap, Situbondo and Batam. Those samples were selected activity and the spectrum to Vibrio using paperdisc diffusion method on double layer agar. Anti Vibrio substances were isolated by liquid partition, column chromatography and thin layer chromatography. The isolated substances then elucidated by color reaction. In Vitro antimicrobial activity (MIC and MBC) were evaluated. Screening based on activity and antagonist spectrum resulted 2nd elected samples 5C and KBa with 5 strain of genus Vibrio bacteria. Active fractions anti Vibrio (5C1-3, KBa1-3) success isolated. Active fractions 5C and KBa indicated positive respond to color reaction, that approx include alkaloid and flavonoid compounds. Active fractions 5C and KBa1 showed bactericidal effect at doses 3,1 and 1,56 mg/ml to V. alginolyticus 26S and V. parahaemolitycus 29S. The identification which has been conducted indicated that R. mucronata leaf sample and wood X. rumphii has some active compounds to words Vibrio bacteria and can to control Vibrio activities.

Kata Kunci : Pengendalian,Vibrio,Ekstrak mangrove,Bacteria Control, Vibrio , Mangrove extracts