Efektivitas Oxytetracycline terhadap infeksi Streptococcus iniae isolat dari Kupang pada ikan Patin (Pangasius sutchi)

SRINOTO, Didik, drh. Surya Amanu, SU

2008 | Tesis | S2 Sain Veteriner

Penelitian ini bertujuan untuk mengetahui Efektivitas Oxytetracycline pada Ikan Patin (Pangasius sutchi) yang diinfeksi bakteri Streptococcus iniae isolat dari Kupang, yang dilakukan di laboratorium Patologi dan Mikrobiologi Fakultas Kedokteran Hewan Universitas Gadjah Mada Yogyakarta. Penelitian dilakukan tahap pertama yaitu penelitian pendahuluan meliputi pemurnian isolat, uji sensitlvitas streptococcus iniae terhadap antibiotik erythromycine, enrofloxacine, amoxycilline, oxytetracycline dan tetracycline dengan metoda Kirby- Bauer dan penentuan Minimum Inhibitory Concentration (MIC) antibiotik terpilih, dengan metode "Tube dillution test". Tahap kedua penelitian utama terdiri 7 kelompok ikan masing-masing 10 ekor. 2 kelompok sebagai kontrol, 1 kelompok tanpa diinfeksi dan tanpa pengobatan antibiotik, dan kelompok kontrol yang lain hanya diinfeksi tanpa pengobatan antibiotik. Sebanyak 5 kelompok perlakuan diinfeksi dengan LCso secara rendaman. Pengobatan dilakukan setelah menunjukkan gejala klinis masing-masing dengan antibiotik 1, 2, 4, 8, 16 kali MIC, secara rendaman 4 jam perhari selama 5 hari. Hasil penelitian, menunjukkan isolat mumi Streptococcus iniae berdasarkan sifat morfologi dan biokimianya. Uji sensitivitas Sreptococcus iniae terhadap antibiotik derajat resisten ditunjukkan pada antibiotik enrofloxacine, erythromycine. tetracycline, amoxycilline, sedangkan derajat sensitif ditunjukkan oxytetracycline. Hasil nilai MIC oxytetracycline terhadap Streptococcus iniae yaitu 4 pg/ml. Pada hasil uji Efektivitas pengobatan 8 kali MIC (32 pg/ml) memberikan kelangsungan hidup 90 %, 1 kali MIC dan 4 kali MIC (4 dan 16 pg/ml) memberikan kelangsungan hidup 70 %, 2 kali MIC (8 pg/ml) memberikan kelangsungan hidup 60% sedangkan 16 kali MIC (64 pg/mt) kelangsungan hidupnya 10%. Perubahan histopatologi ikan diinfeksi S. iniae menunjukkan radang kulit, otot dan usus, kongesti hati, limpa dan ginjal, degenerasi hati dan ginjal. Sedangkan hasil histopatologi ikan diobati dosis 8 x MIC menunjukkan kongesti hati, ginjal dan limpa. Berdasarkan hasil penelitian disimpulkan oxytetracycline dosis 8 x MIC (32 pg/ml) merupakan dosis paling efektif untuk pengobatan S. Iniae pada Ikan Patin (Pangasius sutchi). Kata-kata kunci : Ikan patin {Pangasius sutchi), Efektivitas, Oxytetracycline, Streptococcus iniae.

The purpose of this study is to find out the effectivity of Oxytetracycline on Patin Fish {Pangasius sutchi) infected by the bacteria of isolate Streptococcus iniae from Kupang, done in laboratory of the Pathology and Microbiology, Animal Medicine Faculty, Gadjah Mada University, Yogyakarta. The study is conducted in several stages, i.e. first, a preliminary study that includes isolate purification, test on the effectivity oiStreptococcus iniae on some antibiotics such as erythromycine, enroflaxacine, amoxycilline, oxytetracycline, and tetracycline by using Kirby-Bauer method and determination of the Minimum Inhibitory Concentration (MIC) of selected antibiotic by using a method of "Tube dilution test." Second, primary study consists of 7 groups of fish each of which is 10 tails. Two groups as control, one group as placebo (without being infected and no antibiotic medicine), and other control group is only infected without antibiotic medicine. Five groups of treatment are infected by LC50 submerged. Medicine is conducted after indicating their own clinical symptoms with antibiotic of 1, 2, 4, 8, 16 times of MIC submerged for four hours a day during five days. The result of the study indicates that the pure isolate of Streptococcus iniae is based on the morphological and biochemical properties. Form the test of the sensitivity of Streptococcus iniae on antibiotic, it can be concluded that the rate of resistance is shown at the antibiotics of enroflaxacine, erythromycine, tetracycline and amoxycilline, white the rate of sensitivity is at the antibiotic of oxytetracycline. The result of the MIC value of oxytetracycline on Streptococcus iniae is 4 pg/ml. The result of test for the effectivity of medicine for 8 times of MIC (32 pg/ml) indicates the survival of 90%, 1 time and 4 times of MIC (4 and 16 pg/ml) that of 70%, 2 times of MIC (8 pg/ml) that of 60%, while 16 times of MIC (64 pg/ml) that of 10%. Histopathological change shows that inflammation occurred in skin and heart, inflammation and necrosis in muscle, congestion and degeneration in heart, necrosis, congestion, and degeneration in kidney, and congestion in lymph. Keywords: Patin Fish (Pangsius sutchi), Effectivity of Oxytetracycline, Bacteria of Streptococcus iniae

Kata Kunci : Ikan Patin, Pangasius sutchi,Oxytetracyline, Streptococcus iniae,Patin Fish (Pangsius sutchi), Effectivity of Oxytetracycline