Pengaruh tinopal terhadap Patogenisitas Nucleopolyhedrovirus pada Spodoptera Litura

AMBARWATI, Ma'unah, Dr.Ir. Arman Wijonarko, M.Sc

2007 | Tesis | S2 Ilmu Hama Tumbuhan

Kepekaan larva instar 3, 4 dan 5 ulat grayak, Spodoptera litura (Fabricius) (Lepidoptera : Noctuidae), terhadap NPV diuji dengan metode droplet feeding. Larva berasal dari lapangan yang dikembangbiakkan di laboratorium dengan pakan buatan. Larva Spodoptera litura yang diuji diperlakukan dengan berbagai konsentrasi SlNPV (2 x 103; 2 x 104; 2 x 105; 2 x 106; 2 x 107; 2 x 108; 2 x 109 PIB/ml) yang ditambahkan Tinopal (0,5% dan 1%). Hasil penelitian menunjukkan bahwa dengan penambahan Tinopal 1% berpengaruh signifikan terhadap kematian larva Spodoptera litura instar 3, 4 dan 5. Penambahan Tinopal pada larva instar 3, 4 dan 5 bisa meningkatkan efektivitas NPV sebesar 235; 25117 dan 6,6 juta kali. Pengamatan pada usus tengah larva yang diperlakukan dengan Tinopal menunjukkan bahwa Tinopal secara fisik merusak membran peritropik sehingga diduga membantu masuknya virus kedalam tubuh inangnya.

Susceptibility of the armyworm, Spodoptera litura (Fabricius) (Lepidoptera : Noctuidae) to nucleopolyhedrovirus was evaluated using droplet feeding methods. Spodoptera litura was originally collected from the field in Bantul and has been reared continously in the laboratory using artificial diet. The tested instars were exposed a series concentration of nucleopolyhedrovirus (2 x 103; 2 x 104; 2 x 105; 2 x 106; 2 x 107; 2 x 108; 2 x 109 PIB/ml) which were added by Tinopal 0,5% and 1%. The result indicated that the larval mortality of 3rd, 4th and 5th instars the additional of 1% Tinopal, significantly different in the additional of 1% Tinopal increased the effectiveness of NPV for 235; 25117 and 6,6 million fold. The observation of the larval midgut which was treated by Tinopal, showed that Tinopal physically disrupt the peritrophic membrane. Therefore, it can be suggested that the Tinopal facilitates enterance of NPV to the host insect.

Kata Kunci : Hama Tanaman,Ulat Grayak,NPV Tinopal, Nucleopolyhedrovirus; Spodoptera litura patogenicity; Tinopal