Perubahan reseptivitas endometrium Rattus norvegicus fase estrus akibat pemberian kurkumin

ADIYANTI, Sri Suryo, Prof.dr. Sri Kadarsih Soejono, M.Sc.,Ph.D

2005 | Tesis | S2 Ilmu Kedokteran Dasar

Perubahan Reseptivitas Endometrium Rattus norvegicus Fase Estrus Akibat Pemberian Kurkumin Kajian Tebal Endometrium dan Kemampuan Angiogenesis Kurkumin merupakan bahan alam yang mempunyai aktivitas biologis, salah satunya sebagai antifertilitas, namun belum ada penelitian tentang efek antifertilitas kurkumin terhadap reseptivitas endometrium. Tujuan penelitian ini adalah mengkaji tebal jaringan endometrium dan ekspresi VEGF pada endometrium fase sekresi akibat pemberian kurkumin. Dilakukan penelitian eksperimental post-test randomed group design terhadap 36 ekor Rattus norvegicus galur Sprague Dawley, betina usia 28 hari yang dibagi menjadi 6 kelompok. Kelompok 0 tidak diberi perlakuan untuk dilihat masa pro estrusnya, kelompok 1 sampai 5 diinduksi ovulasi dengan pemberian PMSG dan kemudian diberi perlakuan sebagai berikut: kelompok 1 mendapat larutan CMC (pelarut) , kelompok 2 dengan indometasin ED50, kelompok 3-5 mendapat kurkumin ED50, 4 x ED50 dan 8 x ED50. Perlakuan diberikan per oral, selama 8 hari. Pada hari ke-9 tikus dibunuh untuk diambil uterusnya dan dibuat preparat mikroskopik Hematoksilin Eosin untuk diperiksa ketebalan endometrium dan preparat imunohistokimia untuk diperiksa ekspresi VEGF. Analisis statistik menggunakan ANOVA satu jalan dan uji t menunjukkan perbedaan bermakna pengaruh kurkumin terhadap tebal endometrium, terdapat penurunan ketebalan endometrium yang bermakna antara kelompok pro estrus dengan kelompok CMC (p<0.05) dan kelompok pro estrus dengan kelompok indometasin (p<0.05) Selanjutnya terdapat peningkatan ketebalan endometrium yang bermakna antara kelompok CMC dengan kelompok kurkumin ED50 (p<0.05) dan kelompok indometasin dengan kelompok kurkumin ED50 (p<0.05). Kemudian terdapat perbedaan bermakna pula pengaruh kurkumin dengan ekspresi VEGF. Terjadi peningkatan ekspresi VEGF yang bermakna antara kelompok pro estrus dengan kelompok kurkumin ED50 (p<0.05) maupun kelompok kurkumin 8 x ED50. (p<0.05), kelompok CMC dengan kelompok kurkumin ED50 (p<0.05) maupun kelompok kurkumin 8 x ED50. (p<0.05) dan kelompok indometasin dengan kelompok kurkumin 8 x ED50. (p<0.05). Maka dapat disimpulkan bahwa pemberian kurkumin di awal fase estrus meningkatkan ketebalan endometrium dan meningkatkan angiogenesis yang ditandai dengan peningkatan ekspresi VEGF di akhir fase estrus yang menunjukkan peningkatan reseptivitas endometrium.

Change of Endometrium Receptivity in Estrous Phase of Rattus norvegicus after Curcumin Treatment Study of Endometrium Thickness and Angiogenesis Capability Curcumin is a nature compound that has many biological activities, including anti fertility. There is no study about curcumin effect as fertility regulator on endometrium receptivity. This study aimed to observe endometrium thickness and VEGF expression in secretion phase of endometrium after curcumin treatment. This study was experimental, post-test randomed group design. Subjects were 36 female rats Sprague Dawley Rattus norvegicus, 28 days old, which were divided into 6 groups. Group 0 were not treated in order to see the pro estrus phase. Groups 1 to 5 were ovulated inducted by PMSG and were treated as following: group 1 CMC (solvent), group 2 indomethacin ED50 , groups 3-5 were treated curcumin ED50, 4 x ED50 dan 8 x ED50. Treatment were given per oral for 8 days. On the ninth day uterus, as samples were taken and microscopic HE slides were made to observe the endometrium thickness, immunohistochemistry slides also were made to observe VEGF expression. Statistical analysis using one way ANOVA and t-test showed significant difference of curcumin effect to endometrium thickness. Endometrium thickness significantly decreased in pro estrus group compare to CMC group (p<0.05), pro estrus group compare to indomethacin group (p<0.05), then endometrium thickness significantly increased in CMC group compare to curcumin ED50 group (p<0.05) and indomethacin group compare to curcumin ED50 group(p<0.05). There was also significantly difference of curcumin effect to VEGF expression, VEGF expression significantly increased in pro estrus group compare to curcumin ED50 group (p<0.05) and to curcumin 8 x ED50 group(p<0.05), in CMC group compare to curcumin ED50 group (p<0.05) and to curcumin 8 x ED50 group(p<0.05), and in indomethacin group compare to curcumin ED50 group (p<0.05). Conclusion is curcumin that were given in the beginning of estrous phase increased endometrium receptivity by increasing endometrium thickness and increasing VEGF expression at near the end of secretion phase.

Kata Kunci : Reproduksi,Antifertilitas,Kurkumin,Reseptivitas Endometrium