Hubungan Penyimpanan Terhadap Kadar Glukosa Dalam Platelet Concentrate Di RS Dr. Sardjito Yogyakarta

Ahmad Khoirul Anwar, dr. Teguh Triyono, M. Kes, dr. Umi S. Intansari, M. Kes

2007 | Skripsi | S1 PENDIDIKAN DOKTER

Latar Belakang : Platelet concentrate digunakan dalam transfusi trombosit untuk menyelamatkan hidup. Kualitas platelet concentrate penting diketahui sebelum melakukan transfusi trombosit. Salah satu parameter dalam menentukan viabilitas trombosit dalam platelet concentrate adalah kadar glukosa. Glukosa terlibat langsung dalam metabolisme trombosit dalam menghasilkan energi. Tujuan Penelitian : Penelitian ini bertujuan untuk mengetahui kadar glukosa dalam platelet concentrate selama penyimpanan pada hari ke ;1, 6 dan 8, serta mengkaji hubungan kadar glukosa terhadap lamanya penyimpanan. Cara Penelitian : Penelitian ini menggunakan rancangan penelitian kohort observasional berlokasi di UPTD RS Dr.SardjJito Yogyakarta. Subyek terdiri atas 10 sampel platelet concentrate yang dibuat dari whole blood dan diukur menggunakan metode GOD-PAP pada hari ke 1, 6 dan 8. Hasil : Hasil penelitian menunjukkan rerata kadar glukosa pada hari ke 1 sebesar 410±16,90 mg/dl, hari ke 6 sebesar 363,6±29,24 mg/dl dan harl ke 8 sebesar 333,6±36,19 mg/dl. Kesimpulan : Terjadi penurunan kadar glukosa yang bermakna pada hari ke ;1, 6 dan 8. Terdapat hubungan negatif yang kuat dan bermakna antara hari penyimpanan dengan kadar glukosa. Perlu dilakukan penelitian mengenai parameter metabolisme yang lain dalam menentukan kualitas platelet concentrate seperti ; kadar laktat, oksigen, karbondioksida, pH dan substrat lain yang berperan dalam metabolisme trombosit.

Background : Platelet concentrate has been used in platelet transfusion for life saving. Measuring quality of platelet concentrate is Important before doing platelet transfusion. One of parameter to determine platelet viability in platelet concentrate is glucose concentration, because glucose involved directly in platelet metabolism to induce energy. Objectives : The aim of this research was to determine glucose level in platelet concentrate during 1st, 6st, and 8th day storage and analyze relationship between glucose level and duration of the storage. Methods : This research used observasional cohort method and located in Unit Pelayanan Transfusi Darah (UPTD) RS Dr.Sardjito Yogyakarta. SubjJects consist of 10 platelet concentrate samples that made from whole blood and samples were measured GOD-PAP method in 1st, 6th and 8th day storage. Result : The result showed that mean of platelet count for 1st day was 410±16,90 mg/dl, 6th day was 363±29,24 mg/dl, and 8th day was 333,6±36,19 mg/dl. Conclusions : There was a significant decrease of glucose level in 1st, 6th, and 8th day storage. There was a strong negative correlation between duration of storage and glucose level. Further study were needed to investigate other metabolism variables which influences quality of platelet concentrates such as ; lactic level, oxygen, carbondiokside and the other substance which involve in platelet metabolism.

Kata Kunci : platelet concentrate, glucose, storage, platelet metabolism