Korelasi Netrofil to Lymphocyte Ratio (NLR) dengan Serum Hepsidin pada Pendonor Darah Rutin

Elsina Veronika Samori, Dr. dr. Teguh Triyono, M.Kes., Sp.PK, Subsp.K.V(K), Subsp.B.D.K.T(K) dan Prof. Dr. dr. Usi Sukorini, M.Kes., Sp.PK., Subsp.H.K(K),Subsp.B.D.K.T(K)

2026 | Tesis-Spesialis | S2 Ilmu Patologi Klinik

Latar Belakang: Pendonor darah rutin berisiko mengalami defisiensi zat besi akibat kehilangan darah berulang. Hepsidin merupakan hormon utama pengatur homeostasis besi tubuh yang dipengaruhi oleh kadar inflamasi dan aktivitas eritropoiesis. Neutrophil-to-Lymphocyte Ratio (NLR) merupakan penanda inflamasi sistemik yang menggambarkan keseimbangan antara imun bawaan dan adaptif. Hubungan antara kadar hepsidin dengan NLR pada pendonor darah rutin, masih belum banyak diteliti.

Tujuan: Untuk menganalisis korelasi antara Neutrophil-to-Lymphocyte Ratio (NLR) dengan kadar hepsidin pada pendonor darah rutin

Metode: Penelitian potong lintang menggunakan data pendonor darah rutin di Unit Donor Darah PMI Yogyakarta periode September-Desember 2024 yang memenuhi kriteria inklusi dan eksklusi. Analisis korelasi dilakukan menggunakan uji korelasi Spearman dengan tingkat kemaknaan p < 0>

Hasil: Sebanyak 162 subjek yang memenuhi kriteria dievaluasi, mayoritas berjenis kelamin laki-laki (74,7%) dengan median usia 37 tahun. Rentang frekuensi donor terbanyak adalah 3-4 kali/tahun (50,6%). Nilai median NLR subjek adalah 1,86 (rentang 0,56–5,16) dan median kadar hepsidin adalah 5,90 ng/mL (rentang 0,8-77,1 ng/mL). Analisis statistik menunjukkan nilai koefisien korelasi antara NLR dengan kadar hepsidin sebesar r = -0,045 dengan nilai signifikansi p = 0,252. Garis regresi pada grafik scatter plot yang cenderung datar mengonfirmasi tidak adanya hubungan linier yang signifikan antara kedua variabel tersebut.

Simpulan: Tidak terdapat korelasi yang bermakna secara statistik antara NLR dengan kadar hepsidin pada pendonor darah rutin. Indikator NLR tidak dapat digunakan sebagai cerminan langsung dari kadar hepsidin maupun penanda defisiensi besi pada populasi pendonor darah sehat.

Background: Regular blood donors are at risk of iron deficiency due to repeated blood loss. Hepcidin is the primary hormone regulating iron homeostasis in the body, which is influenced by inflammation levels and erythropoiesis activity. The Neutrophil-to-Lymphocyte Ratio (NLR) is a systemic inflammatory marker that represents the balance between innate and adaptive immunity. The relationship between hepcidin levels and NLR in regular blood donors has not been extensively studied.

Objective: To analyze the correlation between the Neutrophil-to-Lymphocyte Ratio (NLR) and hepcidin levels in regular blood donors.

Method: This cross-sectional study utilized data from regular blood donors at the Indonesian Red Cross (PMI) Blood Donation Unit in Yogyakarta from September to December 2024 who met the inclusion and exclusion criteria. Correlation analysis was performed using Spearman's correlation test with a statistical significance level set at p < 0>

Results: A total of 162 subjects who met the criteria were evaluated, the majority of whom were male (74.7%) with a median age of 37 years. The most frequent donation range was 3-4 times/year (50.6%). The median NLR value of the subjects was 1.86 (range: 0.56-5.16) and the median hepcidin level was 5.90 ng/mL (range: 0.8-77.1 ng/mL). Statistical analysis showed a correlation coefficient between NLR and hepcidin levels of r = -0.045 with a significance value of p = 0.570. The regression line on the scatter plot graph, which tended to be flat, confirmed the absence of a significant linear relationship between the two variables.

Conclusion: There is no statistically significant correlation between the NLR and hepcidin levels in regular blood donors. The NLR marker cannot be used as a direct reflection of hepcidin levels or as an indicator for iron deficiency in a healthy blood donor population.

Kata Kunci : regular blood donor, hepcidin, NLR, iron deficiency