Pemberian Krioprotektan Dimetil Sulfoxida dengan Konsentrasi Berbeda terhadap Motilitas dan Viabilitas Sperma Ikan Nila Merah (Oreochromis sp.)

Venus Cynthia Hapsari Tambunan, Prof. Dr. Ir Murwantoko, M.Si

2026 | Skripsi | BUDIDAYA PERIKANAN

Penelitian ini bertujuan untuk mengetahui pengaruh penambahan krioprotektan Dimetil Sulfoksida (DMSO) dengan konsentrasi berbeda terhadap motilitas dan viabilitas sperma ikan nila (Oreochromis sp.) setelah proses kriopreservasi serta mengetahui konsentrasi DMSO terbaik dalam mempertahankan motilitas dan viabilitas sperma ikan nila selama penyimpanan pada suhu -196°C. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) dengan lima perlakuan, yaitu penambahan ringer tanpa krioprotektansebagai kontrol, ringer + DMSO 2,5%, ringer + DMSO 5%, ringer + DMSO 7,5%, dan ringer + DMSO 10%. Setiap perlakuan terdiri atas tiga ulangan. Parameter yang diamati dalam penelitian ini meliputi kualitas sperma segar, motilitas, dan viabilitas sperma. Kualitas sperma segar diamati berdasarkan warna, konsistensi kekentalan, pH, dan motilitas individu, sedangkan pada sperma yang telah dikriopreservasi dilakukan pengamatan motilitas dan viabilitas. Data kualitas sperma dianalisis secara deskriptif, sedangkan data motilitas dan viabilitas sperma dianalisis menggunakan analisis ragam (ANOVA) yang dilanjutkan dengan uji Tukey’s Honestly Significant Difference (Tukey HSD) dan analisis regresi polinomial ortogonal untuk menentukan konsentrasi optimum DMSO. Hasil penelitian menunjukkan bahwa sperma segar layak digunakan untuk kriopreservasi. Dimetil Sulfoksida (DMSO) berpengaruh nyata (p<0>

This study aimed to evaluate the effect of different concentrations of Dimethyl Sulfoxide (DMSO) as a cryoprotectant on the motility and viability of Nile tilapia (Oreochromis sp.) sperm after cryopreservation and to determine the optimal DMSO concentration for maintaining sperm motility and viability during storage at ?196°C. A Completely Randomized Design (CRD) was employed with five treatments: Ringer's solution without cryoprotectant (control), Ringer's solution supplemented with 2.5%, 5%, 7.5%, and 10% DMSO. Each treatment was performed in triplicate. The observed parameters included fresh sperm quality, sperm motility, and sperm viability. Fresh sperm quality was evaluated based on color, viscosity, pH, and initial sperm motility, whereas post-thaw sperm was evaluated for motility and viability. Fresh sperm quality data were analyzed descriptively, while sperm motility and viability data were analyzed using analysis of variance (ANOVA), followed by Tukey's Honestly Significant Difference (Tukey's HSD) test and orthogonal polynomial regression analysis to determine the optimal DMSO concentration. The results showed that the fresh sperm quality met the requirements for cryopreservation. Dimethyl Sulfoxide (DMSO) had a significant effect (p < 0>

Kata Kunci : kriopreservasi, DMSO, motilitas, viabilitas, ikan nila, dosis optimal