Aktivitas Imunomodulator Ekstrak Bunga Telang (Clitoria ternatea) Terhadap Leukosit dan Diferensial Leukosit sebagai Indikator Respons Imun pada Mencit (Mus musculus)

Fitri Novianti, Prof. Dr. drh. Siti Isrina Oktavia Salasia

2026 | Skripsi | KEDOKTERAN HEWAN

Bunga telang (Clitoria ternatea L.) diketahui mengandung berbagai senyawa bioaktif, seperti flavonoid, antosianin, dan polifenol, yang memiliki aktivitas biologis, termasuk sebagai imunomodulator. Penelitian ini bertujuan untuk mengevaluasi aktivitas imunomodulator ekstrak Clitoria ternatea  terhadap leukosit dan diferensial leukosit sebagai indikator respons imun pada mencit (Mus musculus). Penelitian ini menggunakan mencit jantan galur BALB/c berumur 3 bulan sebanyak 20 ekor dengan berat badan sekitar 20 – 30 gram sebanyak 20 ekor yang dibagi menjadi lima kelompok, yaitu kontrol negatif (akuades), kontrol positif (Stimuno), serta tiga kelompok perlakuan yang diberikan ekstrak Clitoria ternatea  dengan dosis 100 mg/kgBB, 200 mg/kgBB, dan 400 mg/kgBB. Parameter yang diamati meliputi jumlah leukosit total serta diferensial leukosit yang terdiri dari neutrofil, limfosit, dan monosit. Pemeriksaan hematologi dilakukan menggunakan hematology analyzer VetScan HM5. Data dianalisis menggunakan uji ANOVA yang dilanjutkan dengan uji least significant difference (LSD). Hasil penelitian menunjukkan bahwa jumlah leukosit total pada kontrol negatif sebesar 12,8×10?/L, kontrol positif 6,8×10?/L, serta kelompok perlakuan masing-masing sebesar 10,5×10?/L (100 mg/kgBB), 12,2×10?/L (200 mg/kgBB), dan 9,5×10?/L (400 mg/kgBB). Jumlah limfosit pada kontrol negatif sebesar 9,0×10?/L, kontrol positif 3,1×10?/L, serta kelompok perlakuan sebesar 7,9×10?/L, 8,5×10?/L, dan 6,2×10?/L. Sementara itu, jumlah neutrofil berkisar antara 2,3–3,7×10?/L dan monosit antara 0,17–0,23×10?/L pada seluruh kelompok perlakuan. Analisis statistik menunjukkan bahwa pemberian ekstrak Clitoria ternatea berpengaruh signifikan terhadap jumlah leukosit total dan limfosit (p<0>0,05).Berdasarkan hasil penelitian, pemberian ekstrak Clitoria ternatea menunjukkan potensi aktivitas imunomodulator terhadap parameter hematologi, khususnya leukosit total dan limfosit sebagai komponen respons imun.

Butterfly pea (Clitoria ternatea L.) is known to contain several bioactive compounds, including flavonoids, anthocyanins, and polyphenols, which have various biological activities, one of which is as an immunomodulator. This study was conducted to evaluate the immunomodulatory activity of Clitoria ternatea extract on leukocyte and differential leukocyte counts as indicators of immune response in mice (Mus musculus). The study used 20 male BALB/c mice aged 3 months with body weights ranging from 20–30 g. The animals were divided into five groups, consisting of a negative control group (distilled water), a positive control group (Stimuno), and three treatment groups receiving Clitoria ternatea extract at doses of 100 mg/kgBW, 200 mg/kgBW, and 400 mg/kgBW. The observed parameters included total leukocyte count and differential leukocyte counts, namely neutrophils, lymphocytes, and monocytes. Hematological examination was performed using a VetScan HM5 hematology analyzer. The data were analyzed using one-way ANOVA followed by the least significant difference (LSD) test. The results showed that total leukocyte counts in the negative control, positive control, and treatment groups were 12.8×10?/L, 6.8×10?/L, 10.5×10?/L (100 mg/kgBW), 12.2×10?/L (200 mg/kgBW), and 9.5×10?/L (400 mg/kgBW), respectively. Lymphocyte counts in the negative control, positive control, and treatment groups were 9.0×10?/L, 3.1×10?/L, 7.9×10?/L, 8.5×10?/L, and 6.2×10?/L, respectively. Neutrophil counts ranged from 2.3–3.7×10?/L, while monocyte counts ranged from 0.17–0.23×10?/L across all groups. Statistical analysis indicated that administration of Clitoria ternatea extract significantly affected total leukocyte and lymphocyte counts (p<0>0.05). Based on the results of this study, Clitoria ternateaextract showed potential immunomodulatory effects on hematological parameters, particularly total leukocyte and lymphocyte counts, which are involved in the immune response.

Kata Kunci : Clitoria ternatea , imunomodulator, leukosit, diferensial leukosit, Mus musculus, immunomodulator, leukocytes, differential leukocyte count