Identifikasi virus Avian Influenza pada unggas dan produknya dengan metode RT-PCR di Balai besar Karantina Hewan tanjung Perak

DARMINI, Luh, Dr.drh. Soedarmanto Indarjulianto

2005 | Tesis | S2 Sain Veteriner

Penelitian ini bertujuan untuk mengidentifikasi virus Avian influenza menggunakan metode RT-PCR pada unggas dan produknya yang dilalulintaskan melalui Balai Besar Karantina Hewan Tanjung Perak dan dapat memberikan informasi cara-cara penggunaan metode RT-PCR di lapangan. Penelitian ini menggunakan 8 jenis sampel diambil dari komoditas domestik (telur ayam dan DOC), komoditi ekspor (sarang burung wallet, daging ayam beku) dan komoditas impor (Poultry Product Meal (PPM), Feather Chicken Meal (FCM), bebek air dan DOC). Penelitian dilakukan di Laboratorium TDC (Tropical Disease Center) universitas Airlangga Surabaya. Identifikasi virus Avian influenza diawali dengan isolasi RNA dari semua sampel, kemudian dilanjutkan proses RT-PCR dan diakhiri dengan proses gel elektroforesis untuk visualisasi hasil amplifikasi. Hasil penelitian selanjutnya dianalisa secara deskriptif-kualitatif. Dari hasil penelitian ini dapat disimpulkan bahwa sampel dari komoditas unggas dan produknya yang melalui Balai Besar Karantina Hewan Tanjung Perak menunjukkan hasil yang negatif terhadap virus Avian Influenza.

The aim of this research was to identify Avian influenza virus using RT-PCR method on poultry and its products trafficked through the Balai Besar Karantina Hewan Tanjung Perak (BBKH) and also to provide information on RT-PCR method. The research used 8 types of samples from domestic commodities (chicken eggs and DOCs), export commodities (bird nests, freeze chicken meat) and import commodities (Poultry Product Meal (PPM), Feather Chicken Meal (FCM), Water ducks, and DOC). The research was done at the TDC (Tropical Disease Center) Laboratory of the Airlangga University. Identification of Avian influenza virus was initiated by RNA isolation on all samples, then followed by RT-PCR process and finally by gel electrophoresis for visualized amplification results. The data were descriptivelyqualitatively analyzed. Results showed that poultry commodity samples and its products that trafficked at BBKH Tanjung Perak indicated negative results for Avian influenza virus.

Kata Kunci : Penyakit Hewan,Inveksi,Avian Influenza, Avian influenza virus, Poultry and its product, RT-PCR