Pengaruh Pemberian Hormon Analog GnRH-III [D-Lys6, NHEt] terhadap Tingkat Keberhasilan Pemijahan dan Ekspresi fsh-lh pada Ikan Zebra (Danio rerio Hamilton, 1822)

Nur Faizah, Dr. Dini Wahyu Kartika Sari, S.Pi., M.Si.

2026 | Skripsi | BUDIDAYA PERIKANAN

Beberapa jenis ikan budidaya mengalami kendala dalam produksi benih karena sulitnya merangsang pemijahan secara alami, sehingga diperlukan teknologi induksi hormon untuk memacu pematangan gonad dan mempercepat pemijahan. Hormon Gonadotropin-Releasing Hormone (GnRH) berperan penting dalam mengatur sistem reproduksi ikan melalui stimulasi pelepasan Follicle-Stimulating Hormone (FSH) dan Luteinizing Hormone (LH). Penelitian ini bertujuan untuk mengetahui pengaruh pemberian hormon analog GnRH-III [D-Lys⁶, NHEt] terhadap tingkat keberhasilan pemijahan dan ekspresi gen fsh-lh pada ikan zebra (Danio rerio). Penelitian menggunakan rancangan acak lengkap (RAL) dengan tiga perlakuan, yaitu kontrol negatif berupa PBS, hormon analog GnRH-III [D-Lys⁶, NHEt] atau (3-HL), dan Ovaprim sebagai kontrol positif. Parameter yang diamati meliputi persentase keberhasilan pemijahan, waktu laten, serta ekspresi relatif gen fsh dan lh. Analisis data dilakukan secara deskriptif untuk data pemijahan, sedangkan data ekspresi gen fsh-lh dianalisis menggunakan metode Livak (2^???Ct). Hasil penelitian menunjukkan bahwa perlakuan 3-HL menghasilkan persentase keberhasilan pemijahan yang lebih tinggi dibandingkan perlakuan kontrol negatif, meskipun masih lebih rendah dibandingkan kontrol positif. Perlakuan 3-HL juga mampu mempercepat waktu laten pemijahan dengan rata-rata 205 menit, mendekati perlakuan kontrol positif sebesar 201 menit. Analisis ekspresi gen menunjukkan bahwa perlakuan 3-HL menghasilkan ekspresi relatif tertinggi gen lh pada ikan jantan dan betina, serta gen fsh pada ikan jantan, sedangkan ekspresi relatif gen fsh tertinggi pada ikan betina diperoleh pada perlakuan kontrol positif. Hasil penelitian menunjukkan bahwa perlakuan 3-HL bekerja cukup efektif dalam merangsang sistem reproduksi ikan zebra melalui peningkatan ekspresi gen gonadotropin dan percepatan respons pemijahan.

Several cultured fish species encounter constraints in fish seed production due to the difficulty of inducing natural spawning, thus requiring hormonal induction technology to stimulate gonadal maturation and accelerate spawning. Gonadotropin-Releasing Hormone (GnRH) plays an important role in regulating the reproductive system of fish by stimulating the release of Follicle-Stimulating Hormone (FSH) and Luteinizing Hormone (LH). This study aimed to determine the effect of administering a GnRH-III analog hormone [D-Lys⁶, NHEt] on spawning success and the expression of fsh and lh genes in zebrafish (Danio rerio). The study employed a completely randomized design (CRD) with three treatments: a negative control consisting of PBS, a GnRH-III analog hormone [D-Lys⁶, NHEt]/(3-HL), and Ovaprim as a positive control. Observed parameters included spawning success rate, latency period, and relative expression levels of the lh and fsh genes. Spawning data were analyzed descriptively, while gene expression data were analyzed using the Livak (2^???Ct) method. The results showed that the 3-HL treatment resulted in a higher spawning success rate than the negative control treatment, although it remained lower than that of the positive control. The 3-HL treatment also shortened spawning latency time, with an average of 205 minutes, approaching that of the positive control treatment (201 minutes). Gene expression analysis showed that the 3-HL treatment resulted in the highest relative expression of the lh gene in both male and female fish, as well as the highest relative expression of the fsh gene in male fish, whereas the highest relative expression of the fsh gene in female fish was observed in the positive control treatment. These findings indicate that the 3-HL treatment effectively stimulates the reproductive system of zebrafish by enhancing gonadotropin gene expression and accelerating spawning responses.

Kata Kunci : ekspresi gen fsh-lh, GnRH-III, ikan zebra, keberhasilan pemijahan