MODULASI EKSPRESI GEN BARRIER USUS, MORFOLOGI JEJUNUM, DAN PRODUKTIVITAS BROILER FASE STARTER MELALUI PEMBERIAN FERMENTASI CAIR SPIRULINA PLATENSIS

CHRISTIN FLORENSA LALUS, Dr. Ir. Muhsin Al Anas, S.Pt,IPM ; Prof. Dr. Ir. Chusnul Hanim, M.Si., IPM., ASEAN Eng

2026 | Tesis | S2 Ilmu Peternakan

Penelitian ini mengevaluasi efek Spirulina platensis terfermentasi cair (Liquid Fermented Spirulina platensis/LFSP) yang diproduksi melalui ko-fermentasi menggunakan Limosilactobacillus fermentum BN21 dan Bacillus subtilis terhadap performa pertumbuhan awal, perkembangan saluran pencernaan (gastrointestinal tract/GIT), histomorfologi jejunum, serta ekspresi gen mukosa pada broiler fase starter umur 1 hingga 10 hari. Sebanyak 300 ekor ayam broiler jantan umur sehari (Ross 308) dibagi ke dalam tiga perlakuan dengan 10 ulangan kandang per perlakuan dan 10 ekor per kandang, yaitu kontrol yang diberi 0,1 mL larutan phosphate-buffered saline/ekor/hari (CTRL), 0,1 mL LFSP/ekor/hari (FS01), dan 0,2 mL LFSP/ekor/hari (FS02) yang diberikan secara oral. Perlakuan FS01 secara nyata meningkatkan bobot badan akhir dan indeks performa dibandingkan CTRL, tanpa memengaruhi konsumsi pakan secara signifikan (P = 0,426). Perlakuan FS01 juga meningkatkan perkembangan organ saluran pencernaan, menurunkan pH lumen pada seluruh segmen usus halus, serta memperbaiki histomorfologi jejunum secara signifikan, yang ditunjukkan oleh peningkatan tinggi vili, lebar vili, dan rasio tinggi vili terhadap kedalaman kripta (VH:CD) (P < 0>CLDN1, ZO-1, JAM2, OCLN), gen antioksidan (NRF2, GPX1, SOD1, CAT), serta sitokin antiinflamasi (IL-10 dan IL-13), dan secara bersamaan menurunkan ekspresi mediator proinflamasi (IL-18, TNF-?, dan TLR4) (seluruhnya P < 0 xss=removed xss=removed xss=removed>.

This study evaluated the effects of liquid fermented Spirulina platensis (LFSP), produced by co-fermentation with Limosilactobacillus fermentum BN21 and Bacillus subtilis, on early growth performance, gastrointestinal tract (GIT) development, jejunal histomorphology, and mucosal gene expression in starter broilers from day 1 to day 10. A total of 300 day-old male Ross 308 chicks were assigned to three treatments with 10 replicate pens per treatment and 10 birds per pen, control receiving 0.1 mL phosphate-buffered saline/bird/day (CTRL), 0.1 mL LFSP/bird/day (FS01), and 0.2 mL LFSP/bird/day (FS02). All treatments were administered orally via gavage. FS01 significantly increased final body weight and performance index compared with CTRL, without significantly affecting feed intake (P = 0.426). FS01 also improved GIT organ development, reduced luminal pH across all small intestinal segments, and significantly enhanced jejunal histomorphology, as indicated by increased villus height, villus width, and villus height-to-crypt depth ratio (VH:CD) (P < 0>CLDN1, ZO-1, JAM2,OCLN), antioxidant genes (NRF2, GPX1, SOD1, CAT), and anti-inflammatory cytokines (IL-10 and IL-13), while simultaneously downregulating pro-inflammatory mediators (IL-18, TNF-?, and TLR4) (all P < 0 xss=removed xss=removed xss=removed>

Kata Kunci : Broiler, fermentasi, integritas usus, Spirulina platensis, berkelanjutan./ Broiler; fermentation, gut integrity, Spirulina platensis, sustainable