Pengaruh Teknik Pembersihan dan Adhesif pada Gigi Pasca Bleaching Intrakoronal yang Diaplikasi Sodium Askorbat 35% Terhadap Panjang Resin Tag dan Ketebalan Hybrid Layer
Wildan Pratama Aji, Dr. drg. Tunjung Nugraheni, M.Kes., Sp.KG, Subsp. KE; drg. Margareta Rinastiti, M.Kes., Sp.KG. Subsp.KR., Ph.D.
2026 | Tesis-Spesialis | S2 Ilmu Konservasi Gigi
Kristal askorbat yang terbentuk setelah aplikasi gel
sodium askorbat pasca bleaching intrakoronal dapat menghambat penetrasi
bahan adhesif sehingga mempengaruhi panjang resin tag dan ketebalan hybrid
layer yang berperan terhadap kekuatan ikatan restorasi resin komposit.
Penelitian ini bertujuan mengevaluasi pengaruh teknik pembersihan kristal
askorbat dan teknik adhesif pada gigi pasca bleaching intrakoronal
menggunakan hidrogen peroksida 35% yang diaplikasi sodium ascorbate 35%
terhadap panjang resin tag dan ketebalan hybrid layer.
Empat puluh gigi premolar dipotong horizontal untuk
memisahkan mahkota dan akar, kemudian ditanam dalam malam merah. Bleaching intrakoronal
dilakukan menggunakan hidrogen peroksida 35% selama lima hari, diikuti aplikasi
gel sodium askorbat sebanyak dua kali selama lima menit tiap aplikasi. Sampel
dibagi menjadi dua kelompok teknik pembersihan, yaitu agitasi manual
menggunakan microbrush dan nonagitasi manual. Masing-masing kelompok
dibagi lagi menjadi dua subkelompok berdasarkan teknik adhesif: subkelompok A etch-and-rinse
dan subkelompok B self-etch (n=10). Setelah restorasi resin komposit,
sampel dibelah longitudinal bukal-lingual dan diamati ketebalan hybrid layer
dan panjang resin tag dengan SEM pada perbesaran 300×, 500×, dan 1000×. Panjang
resin tag dan ketebalan hybrid layer diukur menggunakan ImageJ
dan dianalisis dengan uji ANAVA dua jalur.
Hasil uji ANAVA dua jalur menunjukkan adanya pengaruh
teknik pembersihan dan teknik adhesif terhadap panjang resin tag dan
ketebalan hybrid layer. Terdapat interaksi antara teknik pembersihan dan
teknik adhesif terhadap ketebalan hybrid layer (p<0>resin tag (p>0,05). Kesimpulan penelitian ini teknik
agitasi manual microbrush meningkatkan ketebalan hybrid layer dan
panjang resin tag. Teknik etch-and-rinse menghasilkan hybrid
layer yang lebih tebal dan resin tag yang lebih panjang dibanding self-etch.
Ascorbate crystals formed
after the application of sodium ascorbate gel following intracoronal bleaching
may inhibit adhesive penetration, thereby affecting resin tag length and hybrid
layer thickness, both of which play crucial roles in the bond strength of resin
composite restorations. This study aimed to evaluate the effect of ascorbate
crystal cleaning techniques and adhesive systems on resin tag length and hybrid
layer thickness in teeth subjected to intracoronal bleaching with 35% hydrogen
peroxide followed by 35% sodium ascorbate application.
Forty premolar teeth were
sectioned horizontally to separate the crown and root, then embedded in red
wax. Intracoronal bleaching was performed using 35% hydrogen peroxide for five
days, followed by application of sodium ascorbate gel twice for five minutes
each. Samples were divided into two cleaning technique groups: manual agitation
using a microbrush and non-agitation. Each group was further subdivided into
two adhesive system subgroups: subgroup A (etch-and-rinse) and subgroup B
(self-etch) (n=10). After composite resin restoration, the samples were
sectioned longitudinally in a buccolingual direction and examined under SEM at
300×, 500×, and 1000× magnifications to assess hybrid layer thickness and resin
tag length. Measurements were performed using ImageJ software and analyzed
using two-way ANOVA.
The two-way ANOVA results
revealed significant effects of both the cleaning technique and adhesive system
on resin tag length and hybrid layer thickness. There was an interaction
between cleaning technique and adhesive system on hybrid layer thickness (p<0>0.05). It can be concluded that manual
agitation using a microbrush increases both hybrid layer thickness and resin
tag length. The etch-and-rinse adhesive system produces a thicker hybrid layer
and longer resin tags compared with the self-etch system.
Kata Kunci : kristal askorbat, agitasi manual microbrush, etch-and rinse, self-etch, hybrid layer, resin tag
