Penambahan Selenium pada Medium Kapasitas In Vitro terhadap Parameter Kinematika Spermatozoa Kambing

Arina Dinar Nurfadilla Sastranegara, Prof. Ir. Diah Tri Widayati, MP., Ph.D., IPU., ASEAN Eng.

2026 | Tesis | S2 Ilmu Peternakan

Penelitian ini bertujuan untuk mengevaluasi pengaruh suplementasi selenium (Se) dalam medium kapasitasi terhadap parameter kinematika spermatozoa dan integritas tudung akrosom spermatozoa kambing selama proses kapasitasi secara in vitro. Penelitian menggunakan semen beku kambing Saanen yang berasal dari Balai Inseminasi Buatan (BIB) Lembang. Semen beku di-thawing pada suhu 37°C selama 30 detik, kemudian dilakukan washing untuk memisahkan spermatozoa dari pengencer dan sisa krioprotektan. Selanjutnya, spermatozoa diinkubasi dalam tiga media kapasitasi, yaitu tanpa penambahan Se sebagai kontrol (T0), penambahan Se sebesar 5 ppm (T1), dan penambahan Se sebesar 10 ppm (T2). Proses kapasitasi dilakukan selama dua dan tiga jam pada suhu 39°C dengan atmosfer 5% CO?. Parameter kinematika spermatozoa dianalisis menggunakan sistem Computer Assisted Sperm Analysis (CASA), meliputi curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), linearity (LIN), straightness (STR), wobble (WOB), amplitude of lateral head displacement (ALH), dan beat cross frequency (BCF), sedangkan integritas tudung akrosom diamati menggunakan pewarnaan Giemsa. Data dianalisis menggunakan One-Way Analysis of Variance (ANOVA) dan dilanjutkan dengan Duncan’s Multiple Range Test (DMRT) pada taraf signifikansi p<0>p<0>p<0>in vitro.

This study aimed to evaluate the effect of selenium (Se) supplementation in the capacitation medium on sperm kinematic parameters and acrosomal cap integrity of goat spermatozoa during the in vitro capacitation process. Frozen-thawed semen from Saanen bucks obtained from the Lembang Artificial Insemination Center (BIB Lembang) was used in this study. The semen was thawed at 37°C for 30 seconds and subsequently washed to separate spermatozoa from the extender and residual cryoprotectants. The spermatozoa were then incubated in three capacitation media: a control medium without Se supplementation (T0), a medium supplemented with 5 ppm Se (T1), and a medium supplemented with 10 ppm Se (T2). Capacitation was performed for two and three hours at 39°C under an atmosphere of 5% CO?. Sperm kinematic parameters were evaluated using a Computer-Assisted Sperm Analysis (CASA) system, including curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), linearity (LIN), straightness (STR), wobble (WOB), amplitude of lateral head displacement (ALH), and beat cross frequency (BCF). Acrosomal cap integrity was assessed using Giemsa staining. Data were analyzed using one-way analysis of variance (ANOVA), followed by Duncan’s Multiple Range Test (DMRT) at a significance level of p<0>p<0>p<0>in vitro capacitation process of goat spermatozoa.

Kata Kunci : Hiperaktivasi, Integritas akrosom, Kinematika spermatozoa, Selenium