KORELASI EKSPRESI PROTEIN NEURON-SPECIFIC ENOLASE DAN TUMOR NECROSIS FACTOR ALPHA MUKOSA KOLON PASKA DEKOMPRESI PADA PENINGKATAN TEKANAN INTRALUMINAL TIKUS ALBINO GALUR WISTAR (Rattus norvegicus)

Fauzan Kurniawan, Dr. dr. Imam Sofii, Sp.B., Subsp. B.D.(K) ; Prof. Dr. dr. Irianiwati, Sp.PA., Subsp. O.G.P.(K)

2026 | Tesis-Spesialis | S2 Ilmu Bedah

Latar Belakang: Peningkatan tekanan intraabdomen (intra-abdominal pressure / IAP) dapat memicu sindrom kompartemen abdomen yang ditandai dengan peningkatan ekspresi protein tumor necrosis factor-alpha (TNF-?) dan neuron-specific enolase (NSE). Tindakan dekompresi berperan dalam menurunkan IAP dan menekan perubahan biokimia tersebut.

Tujuan: Mengetahui korelasi perubahan ekspresi protein NSE dan TNF-? pada mukosa kolon setelah dekompresi pada model peningkatan tekanan intraluminal kolon pada tikus albino galur Wistar (Rattus norvegicus).

Metode: Penelitian ini merupakan studi eksperimental laboratorium dengan rancangan post-test only control design, dilaksanakan di Laboratorium Pusat Antar Universitas Universitas Gadjah Mada, Yogyakarta, pada Juni–Juli 2025. Subjek penelitian terdiri dari 30 ekor tikus albino jantan galur Wistar (berat 170–200 g, usia 2–3 bulan) yang dibagi secara acak menjadi tiga kelompok: dekompresi, tanpa dekompresi, dan kontrol. ekspresi protein NSE dan TNF-? diukur serta dianalisis korelasinya antar kelompok.

Hasil: Dekompresi memberikan pengaruh signifikan terhadap ekspresi protein NSE (r = 0,721; p = 0,019) dan TNF-?, dengan korelasi kuat dibandingkan kelompok tanpa dekompresi (r = 0,803; p = 0,005) dan kelompok kontrol (r = 0,641; p = 0,005). Setelah dekompresi, ekspresi protein NSE meningkat sebesar 51,9?n TNF-? sebesar 40,6%, yang mengindikasikan mekanisme iskemia-reperfusi pascatindakan.

Kesimpulan: Terdapat korelasi ekspresi protein NSE dan TNF-? pada mukosa kolon setelah dekompresi.

Background: Increased intra-abdominal pressure (IAP) can lead to abdominal compartment syndrome, characterized by elevated levels of tumor necrosis factor-alpha (TNF-?) and neuron-specific enolase (NSE). Decompression is an important intervention to reduce IAP and attenuate these biochemical alterations.

Objective: To determine the correlation between changes in NSE and TNF-? levels in colonic mucosa after decompression in a model of increased intraluminal pressure in albino Wistar rats (Rattus norvegicus).

Methods: This laboratory-based experimental study employed a post-test only control design and was conducted at the Pusat Antar Universitas Laboratory, Universitas Gadjah Mada Yogyakarta, from June to July 2025. Thirty male albino Wistar rats (170–200 g, aged 2–3 months) were randomly assigned into three groups: decompression, non-decompression, and control. NSE and TNF-? levels were measured and compared among groups, and correlation coefficients were calculated.

Results: Decompression significantly affected NSE levels (r = 0.721; p = 0.019) and TNF-? levels, with strong correlations observed compared to the non-decompression group (r = 0.803; p = 0.005) and control group (r = 0.641; p = 0.005). Post-decompression analysis demonstrated a 51.9% increase in NSE and a 40.6% increase in TNF-?, suggesting an ischemia-reperfusion mechanism following decompression in intra-abdominal hypertension.

Conclusion: There was a correlation between NSE and TNF-? protein expression in colonic mucosa after decompression.

Kata Kunci : Tekanan intraabdomen, dekompresi, NSE, TNF-?, Rattus norvegicus