EVALUASI EKSTRAK KEMUKUS SEBAGAI ANTIPARASIT SECARA IN VITRO

Delta Wiga Pramana, Prof. Dr. Ir. Kustantinah, DEA., IPU

2026 | Skripsi | ILMU DAN INDUSTRI PETERNAKAN

Penelitian ini bertujuan untuk mengetahui efektivitas penggunaan ekstrak kemukus sebagai agen antiparasit terhadap cacing Haemonchus contortus secara in vitro dengan level yang berbeda. Penelitian ini menggunakan lima perlakuan yaitu kontrol negatif dengan NaCl l0,9%, NaCl 0,9% + 3 mg/ml ekstrak kemukus, NaCl 0,9% + 6 mg/ml ekstrak kemukus, NaCl 0,9% + 9 mg/ml ekstrak kemukus, dan kontrol positif dengan NaCl 0,9% + 3 ml albendazole. Masing-masing perlakuan dilakukan 3 kali ulangan. Variabel yang diamati yaitu kematian cacing (menit ke 0,10, 20, 30, 60, 120, 180, 240) dan daya hambat tetas telur (selama 24 jam). Data yang diperoleh di analisis variansi sesuai rencana acak lengkap pola searah (one-way Anova), berbeda antara rerata diuji menggunakan uji Duncan Multiple Range Test. Hasil analisis menunjukkan bahwa penggunaan ekstrak kemukus 3, 6, 9 mg/ml berpengaruh nyata (P<0>Haemonchus contortus lebih tinggi dibandingkan dengan kontrol negatif serta sama efektifnya dengan albendazole. Penambahan ekstrak kemukus dengan dosis 9 mg/ml berpengaruh nyata dalam meningkatkan mortalitas cacing dewasa hingga 34,72±33,82?lam menit ke-,10, 20, 30, 60, 120, 180, 240 dan persentase hambatan penetasan cacing telur Haemonchus contortus sebesar 86,15±4,26% sehingga berpotensi sebagai anthelmintik alami pengganti albendazole

This study aimed to evaluate the effectiveness of kemukus extract as an antiparasitic agent against Haemonchus contortus worms in vitro at different concentration levels. The experiment consisted of five treatments: a negative control using 0.9% NaCl, 0.9% NaCl supplemented with 3 mg/ml kemukus extract, 0.9% NaCl supplemented with 6 mg/ml kemukus extract, 0.9% NaCl supplemented with 9 mg/ml kemukus extract, and a positive control using 0.9% NaCl combined with 3 ml albendazole. Each treatment was replicated three times. The observed variables included worm mortality at 0, 10, 20, 30, 60, 120, 180, and 240 minutes, as well as egg hatching inhibition over a 24-hour period. The collected data were analyzed using analysis of variance (ANOVA) based on a completely randomized design with a one-way pattern. Differences among treatment means were further tested using Duncan’s Multiple Range Test. The results showed that the application of kemukus extract at concentrations of 3, 6, and 9 mg/ml had a significant effect (P<0>Haemonchus contortus, showing greater effectiveness than the negative control and comparable efficacy to albendazole. The adding of kemukus extract at a dose of 9 mg/ml significantly increased adult worm mortality up to 34,72±33,82% at observation times of 10, 20, 30, 60, 120, 180, and 240 minutes, and achieved an egg hatching inhibition percentage of 86,15±4,26%. Therefore, kemukus extract has the potential to be used as a natural alternative anthelmintic to replace albendazole.

Kata Kunci : Anthelmintik in vitro, daya hambat telur cacing, haemonchus contortus, kemukus.