Hasil: Dosis, waktu, serta interaksi dosis×waktu berpengaruh signifikan terhadap kadar SMN yang terukur (p < 0 class="s1">Kesimpulan: PGV-1 tidak menunjukkan peningkatan kadar protein SMN yang konsisten pada fibroblas SMA dalam kondisi penelitian ini, dan efek yang diamati kemungkinan dipengaruhi oleh sitotoksisitas yang bergantung pada dosis dan waktu. Background: Spinal muscular atrophy (SMA) results from SMN1 loss, leading to reduced SMN protein. Curcumin analogs such as pentagamavunon-1 (PGV-1) may modulate SMN-related pathways. Objective: To assess the effect of PGV-1 on SMN protein levels in fibroblasts from SMA type 1 and type 2 patients. Methods: An in vitro experiment was conducted using SMA type 1, SMA type 2, and normofibroblast cell lines. Cells were treated with PGV-1 (0.75, 1.5, 2.25 µM) for 48 and 72 hours, with DMSO (0.05%) as vehicle control. Viability was assessed by MTT, and SMN protein levels were measured by ELISA. Data were analyzed using two-way ANOVA and Tukey post hoc tests (p < 0>"> Hasil: Dosis, waktu, serta interaksi dosis×waktu berpengaruh signifikan terhadap kadar SMN yang terukur (p < 0 class="s1">Kesimpulan: PGV-1 tidak menunjukkan peningkatan kadar protein SMN yang konsisten pada fibroblas SMA dalam kondisi penelitian ini, dan efek yang diamati kemungkinan dipengaruhi oleh sitotoksisitas yang bergantung pada dosis dan waktu. Background: Spinal muscular atrophy (SMA) results from SMN1 loss, leading to reduced SMN protein. Curcumin analogs such as pentagamavunon-1 (PGV-1) may modulate SMN-related pathways. Objective: To assess the effect of PGV-1 on SMN protein levels in fibroblasts from SMA type 1 and type 2 patients. Methods: An in vitro experiment was conducted using SMA type 1, SMA type 2, and normofibroblast cell lines. Cells were treated with PGV-1 (0.75, 1.5, 2.25 µM) for 48 and 72 hours, with DMSO (0.05%) as vehicle control. Viability was assessed by MTT, and SMN protein levels were measured by ELISA. Data were analyzed using two-way ANOVA and Tukey post hoc tests (p < 0>">
Laporkan Masalah

Effects of Curcumin Analog on Survival Motor Neuron Protein Level in Fibroblast from Spinal Muscular Atrophy Patients

Ghita Alya Ranindya, dr. Dian Kesumapramudya Nurputra, Sp.A., Ph.D., Sp.A.Subsp.Neuro.K; dr. Yogik Onky Silvana Wijaya, Ph.D.

2026 | Skripsi | PENDIDIKAN DOKTER

Latar Belakang: Spinal muscular atrophy (SMA) terjadi akibat kehilangan gen SMN1 yang menyebabkan penurunan kadar protein SMN, dan analog kurkumin seperti pentagamavunon-1 (PGV-1) diduga dapat memodulasi jalur yang berkaitan dengan SMN. Tujuan: Menilai efek PGV-1 terhadap kadar protein SMN pada fibroblas dari pasien SMA tipe 1 dan tipe 2. Metode: Penelitian in vitro dilakukan menggunakan kultur sel fibroblas SMA tipe 1, SMA tipe 2, dan normofibroblas, yang diberi perlakuan PGV-1 (0,75; 1,5; 2,25 µM) selama 48 dan 72 jam dengan DMSO (0,05%) sebagai kontrol; viabilitas sel diukur menggunakan uji MTT dan kadar protein SMN diukur menggunakan ELISA, kemudian data dianalisis dengan two-way ANOVA dan uji lanjut Tukey (p < 0 class="s1">Hasil: Dosis, waktu, serta interaksi dosis×waktu berpengaruh signifikan terhadap kadar SMN yang terukur (p < 0 class="s1">Kesimpulan: PGV-1 tidak menunjukkan peningkatan kadar protein SMN yang konsisten pada fibroblas SMA dalam kondisi penelitian ini, dan efek yang diamati kemungkinan dipengaruhi oleh sitotoksisitas yang bergantung pada dosis dan waktu.

Background: Spinal muscular atrophy (SMA) results from SMN1 loss, leading to reduced SMN protein. Curcumin analogs such as pentagamavunon-1 (PGV-1) may modulate SMN-related pathways. Objective: To assess the effect of PGV-1 on SMN protein levels in fibroblasts from SMA type 1 and type 2 patients. Methods: An in vitro experiment was conducted using SMA type 1, SMA type 2, and normofibroblast cell lines. Cells were treated with PGV-1 (0.75, 1.5, 2.25 µM) for 48 and 72 hours, with DMSO (0.05%) as vehicle control. Viability was assessed by MTT, and SMN protein levels were measured by ELISA. Data were analyzed using two-way ANOVA and Tukey post hoc tests (p < 0>

Kata Kunci : Spinal Muscular Atrophy (SMA), Survival Motor Neuron (SMN), Curcumin analog

  1. S1-2026-492179-abstract.pdf  
  2. S1-2026-492179-bibliography.pdf  
  3. S1-2026-492179-tableofcontent.pdf  
  4. S1-2026-492179-title.pdf