Pengaruh Nanoemulsi Kombinasi Asam Asetat Kedondong (Spondias dulcis), Kitosan, dan Minyak Atsiri Jahe Merah (Zingiber officinale var. rubrum) terhadap Zona Hambat Pertumbuhan Enterococcus faecalis ATCC 29212

Hilwa Azzahra Husnaina Anas, drg. Asikin Nur, M.Kes., Ph.D, Christia Aye Waindy Vega, S.Kp.G., MDSc.

2026 | Skripsi | ILMU KEPERAWATAN GIGI

Kontaminasi Enterococcus faecalis pada dental unit waterline (DUWL) dapat meningkatkan risiko infeksi nosokomial dalam praktik kedokteran gigi. Chlorhexidine sebagai disinfektan standar memiliki efektivitas tinggi, namun penggunaan jangka panjang berpotensi mengganggu keseimbangan mikrobiota oral. Penelitian ini bertujuan mengetahui pengaruh nanoemulsi kombinasi asam asetat buah kedondong (Spondias dulcis), kitosan, dan minyak atsiri jahe merah (Zingiber officinale var. rubrum) terhadap zona hambat pertumbuhan E. faecalis sebagai alternatif disinfektan berbahan alami.

Penelitian eksperimental laboratoris in vitro menggunakan metode difusi agar. Nanoemulsi diformulasikan dari asam asetat kedondong 1%, kitosan 1%, minyak atsiri jahe merah 0,5%, dan Tween 80 melalui homogenisasi dan ultrasonikasi menghasilkan ukuran droplet 688,6 nm. Pengujian aktivitas antibakteri terhadap E. faecalis ATCC 29212 menggunakan tiga kelompok, kontrol negatif (kitosan 1%), kontrol positif (chlorhexidine 0,2%), dan nanoemulsi dengan lima kali pengulangan. Diameter zona hambat diukur menggunakan jangka sorong ketelitian 0,01 mm setelah inkubasi 24 jam suhu 37°C. Analisis data menggunakan One-Way ANOVA dan Post-Hoc LSD.

Hasil analisis One-Way ANOVA dan Post-Hoc LSD menunjukkan perbedaan signifikan antar kelompok (p<0>Enterococcus faecalis.

Contamination of Enterococcus faecalis in dental unit waterlines (DUWL) increases the risk of nosocomial infections in dental practice. Chlorhexidine, as a standard disinfectant, demonstrates high effectiveness, however long-term use may potentially disrupt oral microbiota balance. This study aims to determine the effect of nanoemulsion combining acetic acid from kedondong fruit (Spondias dulcis), chitosan, and red ginger essential oil (Zingiber officinale var. rubrum) on the inhibition zone of E. faecalis growth as a natural-based disinfectant alternative.

This in vitro experimental laboratory study employed the agar diffusion method. The nanoemulsion was formulated from 1% kedondong acetic acid, 1% chitosan, 0.5% red ginger essential oil, and Tween 80 through homogenization and ultrasonication, yielding a droplet size of 688.6 nm. Antibacterial activity testing against E. faecalis ATCC 29212 utilized three groups: negative control (1% chitosan), positive control (0.2% chlorhexidine), and nanoemulsion, with five replications. The inhibition zone diameter was measured using a vernier caliper with 0.01 mm precision after 24-hour incubation at 37°C. Data analysis employed One-Way ANOVA and Post-Hoc LSD tests.

Results of One-Way ANOVA and Post-Hoc LSD test analysis demonstrated significant differences among groups (p<0>

Kata Kunci : nanoemulsi, asam asetat kedondong, kitosan, minyak atsiri jahe merah Enterococcus faecalis, zona hambat