Evaluasi Dosis Limosilactobacillus fermentum terhadap Kualitas Silase Hijauan Jagung (Zea mays L.) dan Rumput Gama Umami (Pennisetum purpureum cv. GU) dengan Aditif Berbeda

MUHAMMAD THOKSYN FURQAN B, Prof. Ir. Nafiatul Umami, S.Pt., M.P., Ph.D., IPM., ASEAN Eng.; Ir. Cuk Tri Noviandi, S.Pt., M.Anim.St., Ph.D., IPM., ASEAN Eng.

2026 | Tesis | S2 Ilmu Peternakan

Penelitian ini bertujuan untuk mengevaluasi jenis aditif terbaik, menentukan dosis optimal Limosilactobacillus fermentum, serta mengkaji interaksi keduanya terhadap kualitas silase campuran hijauan jagung (Zea mays L.) dan rumput Gama Umami (Pennisetum purpureum cv. GU). Penelitian menggunakan rancangan acak lengkap pola  faktorial 3×3 dengan faktor pertama berupa penambahan aditif (tanpa aditif, tepung gaplek 10%, dan dedak padi 10%), serta faktor kedua berupa level inokulum (0, 10³, dan 10? cfu/mL/g silase), masing-masing direplikasi sebanyak 5 kali. Silase dibuat dari hijauan jagung dan rumput GU yang dilayukan hingga kadar air <60>3, asam laktat, asam asetat, asam propionat dan asam butirat), Fleigh score, karakteristik mikroba (BAL), serta KcBK dan KcBO secara in vitro. Data yang diperoleh dianalisis menggunakan statistik anova. kecuali kualitas fisik diuji secara deskriptif. Hasil penelitian menunjukkan bahwa tepung gaplek merupakan aditif paling efektif, ditandai dengan pH lebih rendah (3,42), Fleigh score tertinggi (124), peningkatan BETN, kandungan SK terendah (22,3%), populasi BAL tinggi, serta nilai KcBK dan KcBO terbaik. Level inokulum 10³ cfu/mL/g merupakan dosis optimal karena menghasilkan populasi BAL tertinggi (6,92 log 10 cfu/mL) dan fermentasi yang lebih efisien dibanding dosis lebih tinggi. Interaksi terbaik diperoleh pada kombinasi tepung gaplek dan inokulum 10³ cfu/mL/g, yang memberikan kualitas fisik dan kimia silase paling baik serta fermentasi paling stabil. Kesimpulannya adalah penggunaan tepung gaplek dan inokulum L. fermentum 10³ cfu/mL/g merupakan formula optimal dalam pembuatan silase campuran hijauan jagung dan rumput Gama Umami.

This study aimed to evaluate the most effective additive type, determine the optimal dose of Limosilactobacillus fermentum, and assess the interaction between these factors on the quality of mixed corn forage (Zea mays L.) and Gama Umami grass (Pennisetum purpureum cv. GU ) silage. The experiment used a 3×3 factorial Completely Randomized Design with three additive treatments (no additive, 10?ssava flour, and 10% rice bran) and three inoculant levels (0, 10³, and 10? cfu/mL/g of silage), each replicated five times. The silage was fermented for 90 days and subsequently evaluated for physical quality (color, aroma, texture, and mold presence), chemical composition (DM, OM, CP, CF, EE, and NFE), fermentation characteristics (pH, NH?, lactic acid, acetic acid, propionic acid, and butyric acid), Fleigh score, microbial characteristics (LAB population), and in vitro DMD and OMD. The data obtained were analyzed using ANOVA statistical procedures, except for the physical quality parameters, which were evaluated descriptively. The results showed that cassava flour was the most effective additive, indicated by lower pH (3.42), the highest Fleigh score (124), increased NFE, the lowest crude fiber content (22.3%), high LAB populations, and superior DM and OM digestibility. The inoculant level of 10³ cfu/mL/g was identified as the optimal dose, producing the highest LAB population (6.92 log 10 cfu/mL) and more efficient fermentation than the higher dose. The best interaction occurred with the combination of cassava flour and 10³ cfu/mL/g inoculant, yielding the best physical and chemical qualities and the most stable fermentation. In conclusion, the use of cassava flour and L. fermentum at 10³ cfu/mL/g represents the optimal formulation for producing high-quality silage from mixed corn forage and Gama Umami grass.

Kata Kunci : Silase, Hijauan Jagung, Rumput Gama Umami, Dedak Padi, Tepung Gaplek, Limosilactobacillus fermentum.