Pengaruh Patch Luka Hidrogel Nanokitosan dengan Freeze-Dried Platelet Rich Plasma, Kalsium Karbonat, dan Cinnamaldehyde terhadap Pertumbuhan Candida albicans

Indira Saraswati Setiadi, Dr. drg. Anne Handrini Dewi., M.Kes; drg. Trianna Wahyu Utami, MD.Sc., Ph.D.

2025 | Skripsi | PENDIDIKAN DOKTER GIGI

Candida albicans merupakan salah satu mikroorganisme yang dapat

ditemukan pada luka diabetes dan menyebabkan infeksi luka, gangren,

amputasi bahkan kematian. Patch luka berbasis hidrogel sebagai perawatan

luka modern dikembangkan dengan penambahan komponen zat aktif.

Penelitian ini bertujuan untuk mengetahui pengaruh patch luka hidrogel

nanokitosan dengan penambahan Freeze-dried Platelet Rich Plasma (FD-

PRP), kalsium karbonat dan cinnamaldehyde terhadap pertumbuhan jamur

C.albicans.

Penelitian dilakukan dengan metode disc diffusion. Terdapat tiga

kelompok, yaitu kelompok kontrol positif berupa hydrocolloid dressing by

Dermafix®; kelompok perlakuan yaitu patch luka hidrogel nanokitosan,

Freeze-dried Platelet Rich Plasma (FD-PRP), kalsium karbonat, dan

cinnamaldehyde; dan kontrol negatif berupa base membrane gelatin dan

nanokitosan. Patch dipotong selebar 6 mm dan diletakkan di atas

permukaan cawan petri yang sudah diinokulasi jamur C.albicans.

Pengamatan dilakukan setelah inkubasi selama 24 jam pada suhu 36?.

Perhitungan zona hambat dilakukan dengan mengukur jarak horizontal,

vertikal, dan diagonal pada zona hambat yang terbentuk.

Hasil uji One-way Anova menunjukkan adanya perbedaan signifikan

pada rerata jumlah zona hambat kelompok perlakuan dan kelompok kontrol

negatif. Perbedaan signifikan juga terdapat antara kelompok perlakuan dan

kelompok kontrol positif. Kesimpulan penelitian ini adalah patch luka

hidrogel nanokitosan dengan penambahan kalsium karbonat, Freeze-dried

Platelet Rich Plasma (FD-PRP) dan cinnamaldehyde berpengaruh terhadap

zona hambat pertumbuhan Jamur C.albicans.

Kata Kunci: Patch hidrogel nanoktiosan, Freeze-dried Platelet Rich

Plasma (FD-PRP), Cinnamaldehyde, Kalsium karbonat, Candida albicans.

Candida albicans is a microorganism that can be found in diabetic wounds and

causes wound infections, gangrene, amputation or death. Modern wound care

hydrogel patches are developed added with active components. This study aims to

determine the effect of hydrogel patch based on nanokitosan with the addition of

Freeze-dried Platelet Rich Plasma (FD-PRP), calcium karbonat, and

cinnamaldehyde on the growth of C.albicans.

The research was conducted using the disc diffusion method. There were three

groups: the positive control group, which used hydrocolloid dressing by Dermafix;

the treatment group, consisting of hydrogel patch with nanokitosan, Freeze-dried

Platelet Rich Plasma (FD-PRP), calcium carbonate and cinnamaldehyde; and the

negative control group, consisting of base membrane made of gelatin and

nanokitosan. Patch samples were cut into discs of 6 mm diameter and placed on the

surface of petri dishes inoculated with C.albicans. Observations were carried out

after 24 hours of incubation at 36?. The inhibitin zone was measured by assesing

the horizontal, vertical, and diagnoal distances of the formed inhibition zone.

The result of the one-way ANOVA test showed significant differences in the

mean inhibition zones bertween the treatment group and the negative control group.

Significant differences were also found between the treatment group and the

positive control group. The conclusionof this study is that hydrogel wound patches

containing nanokitosan, calcium carbonate, Freeze-dried Platelet Rich Plasma (FD-

PRP) and cinnamaldehyde influence the inhibition zone of C.albicans growth.

Keyword: Hydrogel nanokitosan patch, Freeze-dried Platelet Rich Plasma (FD-

PRP), Cinnamaldehyde, Calcium Carbonate, Candida albicans.

Kata Kunci : Patch hidrogel nanoktiosan, Freeze-dried Platelet Rich Plasma (FD-PRP), Cinnamaldehyde, Kalsium karbonat, Candida albicans