Pengaruh Penambahan Cinnamaldehyde, Kalsium Karbonat, dan Freeze-dried Platelet Rich Plasma pada Patch Luka Hydrogel Nanokitosan terhadap Pertumbuhan Staphylococcus aureus
Shinta Olivia Simanjuntak, Dr. drg. Anne Handrini Dewi., M. Kes; Dr. drg. Archadian Nuryanti, M.Kes
2025 | Skripsi | PENDIDIKAN DOKTER GIGI
Luka,
khususnya luka diabetik, merupakan luka yang rentan mengalami infeksi sehingga
proses penyembuhannya menjadi lambat. Staphylococcus aureus merupakan
bakteri yang paling sering menginfeksi luka dan sering resisten terhadap
antibiotik sehingga penanganannya menjadi semakin sulit. Perawatan luka yang
umum digunakan saat ini adalah hydrocolloid dressing, tetapi dressing
ini tidak efektif dalam perawatan luka diabetik karena tidak memiliki zat
antibakteri. Alternatif perawatan luka diabetik yang lebih efektif adalah patch
hydrogel nanokitosan karena bersifat biokompatibel dan dapat melepaskan zat
aktif antibakteri.Aktivitas antibakteri patch tersebut masih perlu
ditingkatkan dengan menambahkan agen antibakteri dan penyembuh luka. Penelitian
ini bertujuan untuk mengetahui pengaruh penambahan cinnamaldehyde,
kalsium karbonat(CaCO3), dan Freeze-dried Platelet Rich
Plasma (FD-PRP) pada patch luka hydrogel nanokitosan terhadap
zona hambat pertumbuhan Staphylococcus aureus.
Pengujian aktivitas antibakteri menggunakan metode disc
diffusion dengan mengukur diameter zona hambat di sekitar paper
disc dengan menggunakan jangka sorong. Terdapat
tiga kelompok uji, yaitu kontrol negatif base membrane gelatin
nanokitosan, kelompok perlakuan patch hydrogel nanokitosan dengan
cinnamaldehyde, CaCO3, dan FD-PRP, dan kontrol positif hydrocolloid
dressing Dermafix®. Masing-masing kelompok uji tersebut dipotong
dengan diameter 6 mm lalu diletakkan di atas paper disc. Pengujian
dilakukan pengulangan sebanyak lima kali.
Data yang diperoleh dianalisis dengan One-way ANOVA. Hasil penelitian menunjukkan bahwa terdapat perbedaan rata-rata diameter zona hambat Staphylococcus aureus yang signifikan antara kelompok kontrol dan perlakuan (p=0,038). Kesimpulan penelitian ini adalah penambahan cinnamaldehyde, CaCO3, dan FD-PRP pada patch luka hydrogel nanokitosan memiliki pengaruh terhadap zona hambat pertumbuhan Staphylococcus aureus.
Wounds,
particularly diabetic wounds, are highly susceptible to infection, which can
significantly delay the healing process. Staphylococcus aureus is one of
the most frequently isolated pathogens in wound infections and is often
resistant to multiple antibiotics, presenting a considerable challenge in
clinical management. Hydrocolloid dressings are commonly used as wound care,
but they are ineffective in treating diabetic wounds due to the absence of
antibacterial agents. A more effective alternative for diabetic wound
management is nanochitosan hydrogel patches, as they are biocompatible and
capable of delivering antibacterial active substances. Nevertheless, the
antibacterial activity of nanochitosan hydrogel patches still needs to be
improved by adding additional antimicrobial and wound-healing agents. This
study aimed to determine the effect of adding cinnamaldehyde, calcium carbonate
(CaCO?), and Freeze-dried Platelet Rich Plasma (FD-PRP) to nanochitosan
hydrogel wound patches on the inhibition zone of Staphylococcus aureus.
The antibacterial activity was tested using the disc diffusion method by measuring the diameter of the inhibition zone around the paper disc with a sliding caliper. There were three groups: a negative control consisting of the gelatin nanochitosan base membrane, a treatment group consisting of the nanochitosan hydrogel patch containing cinnamaldehyde, CaCO?, and FD-PRP, and a positive control consisting of Dermafix® hydrocolloid dressing. Each test group was cut into a 6 mm diameter piece and placed on top of the paper disc. The test was repeated five times.
The data obtained were analyzed using one-way ANOVA. The results showed a significant difference in the average inhibition zone diameter between the control and treatment groups (p=0.038). In conclusion, the addition of cinnamaldehyde, CaCO?, and FD-PRP to nanochitosan hydrogel wound patches has a significant effect on the inhibition zone of Staphylococcus aureus.
Kata Kunci : patch hydrogel nanokitosan, cinnamaldehyde, kalsium karbonat, Freeze-dried Platelet Rich Plasma, Staphylococcus aureus, nanochitosan hydrogel patch, calcium carbonate
