Pengaruh Pemberian Selenium Nanopartikel (SeNP) terhadap Kualitas Spermatozoa dan Hormon Reproduksi Kelinci New Zealand White

Ajeng Hen Mahardika, Prof. drh. Aris Junaidi, Ph.D.

2025 | Tesis | S2 Sain Veteriner

Selenium (Se) merupakan salah satu mineral esensial yang dapat berperan sebagai kofaktor antioksidan endogen yang menunjang berbagai fungsi biologis, termasuk sistem reproduksi. Suplementasi selenium dalam bentuk nanopartikel semakin banyak dilakukan karena bioavailabilitasnya yang lebih tinggi dan efek fisiologis yang lebih kuat dibandingkan bentuk konvensional. Tujuan penelitian yaitu mengetahui pengaruh pemberian SeNP terhadap kualitas spermatozoa (motilitas, viabilitas, membran akrosom, dan PMI), hormon reproduksi (testosteron dan FSH), serta status antioksidan (GPx dan MDA) pada kelinci jantan. Penelitian ini menggunakan hewan coba kelinci New Zealand White jantan dengan model Rancangan Acak Lengkap (RAL). Total sampel sebanyak 30 ekor kelinci berumur ±8 minggu dan berat badan ±1 kg. Hewan coba dibagi rata menjadi 3 kelompok perlakuan yaitu K (kontrol), Se (selenium dosis 0.5 mg/kg BB), dan SeNP (selenium nanopartikel dosis 0,5 mg/kg BB). Perlakuan diberikan selama 60 hari, kemudian kelinci di euthanasia. Darah dikoleksi untuk keperluan analisis hormon reproduksi dan status antioksidan, testis dan epididimis dikoleksi untuk analisis kualitas spermatozoa. Data yang diperoleh akan dianalisis secara statistik menggunakan statistik IBM SPSS dengan uji ANOVA dan uji post hoc Tukey. Hasil stastistik menunjukkan bahwa suplementasi SeNP secara signifikan (p<0>New Zealand White jantan.

Selenium is an essential mineral that functions as a cofactor of endogenous antioxidants and supports various biological processes, including the reproductive system. Supplementation of selenium in the form of nanoparticles has been increasingly utilized due to its higher bioavailability and stronger physiological effects compared to conventional forms. The aim of this study was to determine the effect of SeNP administration on sperm quality (motility, viability, acrosome membrane, and plasma membrane integrity), reproductive hormones (testosterone and FSH), and antioxidant status (GPx and MDA) in male rabbits. This study used male New Zealand White rabbits in a Completely Randomized Design model. A total of 30 rabbits aged ±8 weeks and weighing ±1 kg were used. The animals were evenly divided into three treatment groups: K (control), Se (selenium at a dose of 0,5 mg/kg BW), and SeNP (selenium nanoparticles at a dose of 0,5 mg/kg BW). Treatments were administered for 60 days, after which the rabbits were euthanized. Blood samples were collected for reproductive hormone and antioxidant status analyses, while the testes and epididymis were collected for sperm quality analysis. The data obtained were statistically analyzed using IBM SPSS software with ANOVA and Tukey post hoc tests. The statistical results showed that SeNP supplementation significantly (p<0>

Kata Kunci : selenium nanopartikel, kelinci New Zealand White jantan, hormon reproduksi, kualitas spermatozoa, antioksidan