PERBANDINGAN PENGARUH INJECTABLE PLATELET-RICH FIBRIN DAN ADVANCED PLATELET-RICH FIBRIN TERHADAP EKSPRESI MATRIX METALLOPROTEINASE-9 PADA PERGERAKAN GIGI ORTODONTI KELINCI

Eliza Avianty, Dr. drg. Cendrawasih Andusyana Farmasyanti, M.Kes., Sp.Ort(K) ; Dr. drg. Andi Triawan, Sp.Ort(K)

2025 | Tesis-Spesialis | S2 Ortodonsia

Matrix metalloproteinase-9 adalah satu biomarker dalam pergerakan gigi ortodonti. Ekspresi MMP-9 oleh osteoklas berperan dalam degradasi matriks ekstraseluler selama proses resorpsi dan remodeling tulang. Penelitian ini bertujuan untuk menganalisis perbandingan pengaruh injeksi i-PRF dan a-PRF terhadap ekspresi MMP-9 pada sisi tertekan pergerakan gigi ortodonti kelinci.

Empat puluh lima kelinci dibagi secara acak menjadi 3 kelompok (n=15): kontrol, i-PRF, dan a-PRF. Peranti ortodonti open coil spring dengan kekuatan gaya 50cN dipasang diantara braket insisivus rahang bawah. Aplikasi i-PRF dan a-PRF dilakukan dengan cara injeksi secara intrasulkular pada sulkus gingiva area kompresi setiap 7 hari. Ekspresi MMP-9 dianalisis secara imunohistokimia pada hari ke-0, 3, 7, 14, dan 21.

Hasil penelitian menunjukkan ekspresi MMP-9 meningkat secara signifikan (p<0>

Matrix metalloproteinase-9 is one of the biomarkers involved in orthodontic tooth movement. Expression of MMP-9 by osteoclasts plays important role in extracellular matrix degradation during bone resorption and remodeling. This study aims to analyze the comparative effects of i-PRF and a-PRF injections on MMP-9 expression in compression side during orthodontic tooth movement in rabbits.

Forty-five rabbits were randomly divided into three groups (n=15): control, i-PRF, and a-PRF. Orthodontic force of 50cN was applied to the lower incisors using open coil spring between lower incisor bracket. The i-PRF and a-PRF were gently injected into the distal gingival sulcus every seven days. The MMP-9 expression was examined using immunohistochemistry staining on days 0, 3, 7, 14, and 21.

The results of the study showed that MMP-9 expression increased significantly (p<0>

Kata Kunci : i-PRF, a-PRF, MMP-9, pergerakan gigi ortodonti