PENGARUH KONSENTRASI EKSTRAK KULIT NANAS (Ananas comosus (L.) Merr.) DALAM DENTURE CLEANSER TERHADAP PERLEKATAN MUCIN DAN Candida albicans PADA RESIN AKRILIK

Asa Karina, Dr. drg. Dyah Irnawati, M.S.; Prof. Dr. drg. Siti Sunarintyas, M.Kes

2025 | Tesis | S2 Kedokteran Gigi

    Resin akrilik digunakan sebagai material basis gigi tiruan karena estetika yang baik, namun tidak bersifat antimikroba, sehingga mudah dilekati denture plaque. Kulit nanas mengandung zat yang bersifat antimikroba. Tujuan penelitian ini untuk mengetahui pengaruh konsentrasi ekstrak kulit nanas (Ananas comosus (L.) Merr.) dalam denture cleanser terhadap perlekatan mucin dan Candida albicans (C. albicans) pada resin akrilik.

    Bahan utama penelitian adalah resin akrilik polimerisasi panas dan kulit nanas. Sampel resin akrilik berbentuk disk (diameter 12 mm dan tebal 2 mm) sebanyak 72 buah (n=6). Denture cleanser dengan zat aktif ekstrak kulit nanas dibuat dengan konsentrasi 0%, 15%, 20%, 25%, 30%, dan 35%. Sampel resin akrilik dikontakkan dengan mucin selama 1 jam, direndam dalam denture cleanser selama 8 jam. Mucin pada resin akrilik dirontokkan dan dihitung konsentrasi dengan metode optical density (mg/ml). Koloni C. albicans yang melekat dirontokkan dari resin akrilik dihitung pada media Sabouraud Dextrose Agar (SDA) dengan colony counter (CFU/ml). Data dianalisis menggunakan ANAVA satu jalur dan post-hoc Tamhane0,05.

    Rerata konsentrasi mucin tertinggi pada konsentrasi 0% sebesar 0,757 mg/ml, sedangkan rerata terendah pada konsentrasi 35% sebesar 0,030 mg/ml. Rerata koloni C. albicans tertinggi pada konsentrasi 0% sebesar 214,50 x 104 CFU/ml, sedangkan rerata terendah pada konsentrasi 35% sebesar 1,33 x 104 CFU/ml. Hasil uji ANAVA satu jalur menunjukkan konsentrasi ekstrak kulit nanas dalam denture cleanser berpengaruh signifikan terhadap perlekatan mucin dan C. albicans pada resin akrilik (p<0>mucin dan C. albicans antar semua kelompok (p<0>0,05). Kesimpulan penelitian ini konsentrasi ekstrak kulit nanas (Ananas comosus (L.) Merr.) dalam denture cleanser menurunkan perlekatan mucin dan C. albicans pada resin akrilik.

    Acrylic resin is commonly used as a denture base material because of its good aesthetics, but it is not antimicrobial, so it is easily attached to denture plaque. Pineapple peel contains substances that are antimicrobial. The purpose of this study was to determine the effect of pineapple peel extract (Ananas comosus (L.) Merr.) concentration in denture cleanser on mucin and Candida albicans (C. albicans) adhesion to acrylic resin.

    The main materials of the study were hot polymerized acrylic resin and pineapple peel. The acrylic resin samples were in the form of discs (diameter 12 mm and 2 mm thick) as many as 72 pieces (n=6). Denture cleanser with active substance of pineapple peel extract was made with concentrations of 0%, 15%, 20%, 25%, 30%, and 35%. Acrylic resin samples were contacted with mucin for 1 hour, soaked in denture cleanser for 8 hours. Mucin on the acrylic resin was removed and the concentration was calculated using the optical density method (mg/ml). The attached C. albicans colonies were removed from the acrylic resin and counted on Sabouraud Dextrose Agar (SDA) media with a colony counter (CFU/ml). Data were analyzed using one-way ANOVA and post-hoc Tamhane0.05.

    The highest mean mucin concentration in concentration of 0% was 0.757 mg/ml, while the lowest mean was in concentration of 35% at 0.030 mg/ml. The highest mean C. albicans colony in concentration of 0% was 214.50 x 104 CFU/ml, while the lowest mean was in concentration of 35% at 1.33 x 104 CFU/ml. The results of the one-way ANOVA test showed that the concentration of pineapple peel extract in denture cleanser had a significant effect on the attachment of mucin and C. albicans to acrylic resin (p<0>0.05). The conclusion of this study is that the concentration of pineapple peel extract (Ananas comosus (L.) Merr.) in denture cleanser reduces the attachment of mucin and C. albicans to acrylic resin.

Kata Kunci : C. albicans, denture cleanser, kulit nanas, mucin, resin akrilik