Preformulation and Stability Test of Tamanu Oil Nanoemulsion Serum Spray Preparation as an Anti-Aging Agent Candidate

Nailah Nafisah Najma, Dr. Adhyatmika, M.Biotech., Apt.

2025 | Skripsi | FARMASI

Penuaan kulit adalah proses alami yang dapat diperlambat dengan antioksidan seperti yang terdapat dalam minyak Tamanu. Untuk meningkatkan stabilitas dan efektivitasnya, minyak Tamanu diformulasikan ke dalam bentuk semprotan serum nanoemulsi menggunakan Self-Nanoemulsifying Drug Delivery System. Penelitian ini bertujuan untuk mengoptimalkan komposisi eksipien pada serum semprot minyak Tamanu serta mengevaluasi dampak nanoemulsi terhadap stabilitas formulasi. Optimasi dilakukan menggunakan Simplex Lattice Design pada perangkat lunak Design-Expert v23.1 dengan memvariasikan komposisi Tween 80 dan Polyethylene Glycol 400. Formula optimum dievaluasi melalui respons Z-average, Polydispersity Index, zeta potential, dan transmitansi nanoemulsi. Uji stabilitas dilakukan melalui uji beku-cair (freeze-thaw) serta pengujian karakterisasi meliputi sifat organoleptik, pH, viskositas, dan aktivitas antioksidan. Analisis statistik dilakukan menggunakan IBM SPSS v30.0. Formula optimum mengandung 4,846 g Tween 80 dan 1,154 g Polyethylene Glycol 400. Data eksperimental mengonfirmasi prediksi model terhadap respons. Aktivitas antioksidan sedikit menurun setelah formulasi dengan nilai IC?? dari 1312,59 ± 21,87 menjadi 1905,99 ± 53,69 ppm, yang menunjukkan keberhasilan enkapsulasi. Formula tetap stabil selama siklus uji beku-cair, dengan hanya perbedaan pH yang kecil. Kesimpulannya, formula optimum serum semprot nanoemulsi minyak Tamanu menunjukkan stabilitas yang baik dan mempertahankan aktivitas antioksidan, mendukung potensinya sebagai produk perawatan kulit anti-penuaan.

Skin aging is a natural process that can be delayed with antioxidants such as those found in Tamanu oil. To enhance its stability and effectiveness, Tamanu oil was formulated into a nanoemulsion serum spray using the Self-Nanoemulsifying Drug Delivery System. This study aimed to optimize the excipient composition for Tamanu oil serum spray and assess the impact of nanoemulsion on formulation stability. Optimization was conducted using Simplex Lattice Design in Design-Expert v23.1 by varying Tween 80 and Polyethylene Glycol 400 composition. The optimum formula was evaluated through the responses of Z-average, Polydispersity Index, zeta potential, and nanoemulsion transmittance. The stability test was conducted by freeze-thaw test along with characterization tests, including organoleptic properties, pH, viscosity, and antioxidant activity. Statistical analysis was performed using IBM SPSS v30.0. The optimal formula contained 4.846 g Tween 80 and 1.154 g Polyethylene Glycol 400. Experimental data confirmed model predictions for the responses. Antioxidant activity decreased slightly post-formulation with IC?? value from 1312.59 ± 21.87 to 1905.99 ± 53.69 ppm, suggesting successful encapsulation. The formula remained stable across freeze-thaw test cycles, with only a minor pH difference. In conclusion, the optimum formula of Tamanu oil nanoemulsion serum spray showed good stability and preserved antioxidant activity, supporting its potential as an anti-aging skincare product.

Kata Kunci : Antioxidant, encapsulation, freeze-thaw, self-nanoemulsifying drug delivery system, simplex lattice design