Pengaruh Pemberian Ekstrak Kasar Jamur Tiram dan Probiotik Lactococcus formosensis JAL37 terhadap Sistem Kekebalan Non-Spesifik Seluler Ikan Nila Merah (Oreochromis sp.)
Iqbal Maulana, Indah Istiqomah, S.Pi., MSi., Ph.D.
2025 | Skripsi | BUDIDAYA PERIKANAN
Ikan nila merah ( Oreochromis sp.) merupakan komoditas ikan air tawar yang sangat banyak diminati, namun budidayanya masih rentan terhadap serangan penyakit. Penelitian ini bertujuan untuk memberikan pemberian probiotik Lactococcus formosensis JAL37 dan ekstrak kasar jamur tiram serta kombinasi keduanya (sinbiotik) terhadap sistem imun non-spesifik seluler ikan nila merah selama 60 hari perlakuan. Hasil pengamatan menunjukkan bahwa pemberian perlakuan selama 30 hari meingkatkan secara signifikan nilai hematokrit, konsentrasi glukosa darah, aktivitas fagositosis, jumlah limfosit, serta peningkatan proporsi eusinofil. Setelah masa perawatan yang berlangsung selama 60 hari, peningkatan lebih lanjut dicatat pada parameter hematokrit, aktivitas fagositosis, indeks fagositosis, jumlah limfosit, dan eusinofil. Analisis lebih lanjut mengenai efek interaksi antara probiotik dan ekstrak kasar jamur menunjukkan adanya perbedaan yang signifikan pada beberapa parameter imun seluler, yaitu jumlah hematokrit, aktivitas fagositosis, dan jumlah limfosit.
Red tilapia (Oreochromis sp.) is a freshwater fish commodity that is very much in demand, but its cultivation is still vulnerable to disease attacks. This study aims to determine the provision of probiotics Lactococcus formosensis JAL37 and oyster mushroom crude extract and a combination of both (synbiotic) on the cellular non-specific immune system of red tilapia during 60 days of treatment. The results showed that 30 days of treatment significantly increased the haematocrit value, blood glucose concentration, phagocytosis activity, lymphocyte count, and increased the proportion of eusinophils. After the 60-day treatment period, further increases were recorded in haematocrit, phagocytic activity, phagocytic index, lymphocyte count, and eusinophil parameters. Further analysis of the interaction effect between probiotics and mushroom crude extract showed significant differences in several cellular immune parameters, namely haematocrit count, phagocytosis activity, and lymphocyte count.
Kata Kunci : imun, Lactococcus, nila, ekstrak kasar jamur, sinbiotik