Pengaruh Mutagenesis Sinar UV-C terhadap Produksi Lipid pada Kapang Mucor irregularis Stchigel, Cano, Guarro & E.Alvarez JR 1.1

Adriana Tita Suryawati, Dr. Miftahul Ilmi, M.Si.

2025 | Tesis | S2 Biologi

Oleaginous fungi menjadi salah satu subjek untuk memenuhi peningkatan kebutuhan asam lemak yang tidak diiringi pemenuhan sumber daya lain seperti minyak nabati dan hewani. Mucor irregularis JR 1.1 mampu mengakumulasi lipid hingga mencapai 43,46% menggunakan glukosa sebagai sumber karbon utama. Penggunaan ethanol+H2O2 dan litium klorida dapat menjadi agen selektif dalam menentukan strain mutan potensial dalam produksi lipid. Analisis penelitian dilakukan untuk mengetahui pengaruh mutagenesis sinar UV-C pada produksi lipid strain mutan yang diseleksi menggunakan agen selektif tersebut. Variasi paparan radiasi sinar UV-C (30 Watt) dengan interval waktu 30 detik hingga mencapai persentase kematian sel 95%. Kemudian seleksi strain mutan dengan agen selektif pada konsentrasi H2O2+ethanol (mmol/L):% v/v) (1:1) dan litium klorida (%) (interval 0,5%). Pengaruh radiasi sinar UV-C 180 detik meningkatkan produksi lipid strain mutan M51 (LiCl) hingga 2,46x dibandingkan strain wildtype dengan persentase lipid 35,46±2,36%. Analisis profil asam lemak menggunakan GC-MS menunjukkan adanya perubahan komposisi dan persentase pada strain wildtype dan mutan Mucor irregularis JR 1.1 akibat pengaruh mutagenesis sinar UV-C. Jenis asam lemak yang mengalami peningkatan persentase meliputi asam oleat (M1) 28,67?n asam stearat (M51) 28,75%. 

Oleaginous fungi are one of the subjects to meet the increasing need for fatty acids that are not accompanied by other resources such as vegetable and animal oils. Mucor irregularis JR 1.1 is able to accumulate lipids up to 43,46% using glucose as the main source of carbon. The use of H2O2+ethanol and litium chloride (LiCl) may be selective agents in determining potential mutan strains in lipid production. The research analysis was conducted to determine potential mutan strains in lipid production. The research analysis was conducted to determine the effect of UVC light mutagenesis on the lipid production of lipid strains of mutan selected using these selective agents. Variation in exposure to UV-C radiation (30 Watt) with a time interval of 30 seconds untul it reaches a 95?ll death percentage. Then mutant strain selection with selective agents at the concentraitons of H2O2+ethanol (mmol/:% v/v) (1:1) and lithium chloride (%) (0,5% interval). The effect of UV-C radiation (180 seconds) increased the lipid production of the mutan strain M51 (LiCl) by up to 2,46x compared to the WT1 wildytpe strain with a lipid percentage of 35,46±2,36%. Anaysis of fatty acid profiles using GC-MS showed changes in composition and persentage in wildtype and mutant strains of Mucor irregularis JR 1.1 by the UV-C mutagenesis. The types of fatty acids showed in an increase in persentages including oleic acid (M1) 28,67% and stearic acid (M51) 28,75%. 

Kata Kunci : Lipid, mutagenesis UV-C, agen selektif, oleaginous fungi, GC-MS