PENGARUH SUPLEMENTASI HORMON GONADOTROPIN DALAM MEDIUM TERHADAP MATURASI OOSIT IN VITRO KAMBING BLIGON

Arina Dinar Nurfadilla Sastranegara, Prof. Ir. Diah Tri Widayati, MP., Ph.D., IPM.

2025 | Skripsi | ILMU DAN INDUSTRI PETERNAKAN

Penelitian ini dilakukan untuk mengetahui pengaruh suplementasi hormon gonadotropin dalam medium terhadap maturasi oosit in vitro kambing Bligon. Penelitian ini dilakukan di Laboratorium Fisiologi dan Reproduksi Ternak Fakultas Peternakan Universitas Gadjah Mada Yogyakarta dengan menggunakan ovarium kambing Bligon yang diperoleh dari rumah porong hewan (RPH) sekitar Yogyakarta. Ovarium dibawa dari RPH menggunakan larutan NaCl fisiologis berusuhu 35-40°C. Ovarium selanjutnya dilakukan aspirasi menggunakan syringe berisi flushing medium. Oosit grade A dan B yang diperoleh dilakukan pencucian 3 kali dengan flushing medium dan didistribusikan ke dalam 50 µl medium maturase yang berbeda, yaitu tissue culture medium tanpa suplementasi dan tissue culture medium + 50 IU hormon gonadotropin. Tissue culture dish selanjutnya ditutup dengan mineral oil dan diinkubasi dalam inkubator pada suhu 39°C, kelembaban udara 95%, dan kadar CO₂ 5% selama 22-24 jam. Maturasi oosit diukur berdasarkan ekspansi sel kumulus. Data angka maturasi dianalisa statistic menggunakan analisa Independent Sample T-test dengan SPSS 30, kualitas maturasi oosit dianalisa secara kualitatif. Hasil penelitian menunjukkan bahwa penambahan hormon gonadotropin berpengaruh nyata terhadap tingkat maturasi oosit in vitro (P<0>mature oosit kambing Bligon lebih baik dibandingkan dengan culture medium tanpa suplementasi. 

This research was conducted to determine the effect of gonadotropin hormone supplementation on maturation medium on the in vitro oocyte maturation of Bligon goat. This research was conducted at the Physiology and Animal Reproduction Laboratory, Faculty of Animal Science, Gadjah Mada University, Yogyakarta, using Bligon goat ovaries obtained from slaughterhouses around Yogyakarta. The ovaries were transported from the slaughterhouses using physiological NaCl solution at a temperature of 35-40°C. The ovaries aspirated using a syringe filles with flushing medium. The recovered Grade A and B oocytes were washed three times in flushing medium and were then transferred into 50 µl of different maturation media; tissue culture medium without and with 50 IU gonadotropin hormone supplementation. Finally, the culture dishes were overlaid with mineral oil and further incubated at 39°C with 95% humidity and 5% CO2 for 22–24 hours. Oocyte maturation was determined by cumulus cell expansion. Data for the maturation rates were analyzed by the Independent Sample T-test with SPSS 30, and oocyte maturation quality was measured qualitatively. The addition of gonadotropin hormone showed a significant difference in the process of in vitro maturation of oocytes (P<0>

Kata Kunci : Hormon gonadotropin, Medium maturasi, Oosit kambing Bligon, Maturasi oosit in vitro, Ekspansi sel kumulus