Skrining Bahan Aktif Antibakteri untuk Kosmetika dari Beberapa Ekstrak Kulit Batang Tumbuhan Kawasan Borneo Berdasarkan Aktivitas terhadap Pseudomonas aeruginosa
Salsabila, Prof. Dr.rer.nat. apt. Triana Hertiani, M.Si.
2025 | Skripsi | FARMASI
Plants contain bioactive compounds that can be used as natural cosmetic ingredients. Indonesia has approximately 25,000 types of flora or more than 10% of plant species worldwide. Of these, 155 species are endemic to Borneo. Therefore, it is necessary to conduct research on plants in the Kalimantan region to see the potential as active cosmetic ingredients based on antibacterial activity against Pseudomonas aeruginosa.
Phytochemical screening using the TLC method using silica gel 60 F254 as the stationary phase and methanol:formic acid (7:3:3:1) v/v as the mobile phase. Visualization was carried out using UV light at 254 nm, 366 nm, and visible light; in addition, anisaldehyde-sulfuric acid spray reagents, Dragendroff, sitroborate, and Lieberman-Burchard were used as spot visualizers. Overlay bioautography tests were carried out to determine the group of active compounds in the extract that had antibacterial activity.
A total of 11 methanol extracts of the material were tested for antibacterial activity using the disc diffusion method and continued with the microdilution method to determine the Minimum Inhibitory Concentration (MIC) value. The results of the antibacterial test using the disc diffusion method showed antibacterial activity in the extracts of Macaranga conifora and Triomma malaccensis. The microdilution test with a concentration of 1.6 mg/ml obtained an inhibition value in Macaranga conifora of 42.1% and in Dilenia excelsa of 57.6%. The results obtained from the eleven samples were positive for flavonoids and terpenoid-steroids. The results of the TLC-Bioautography test using the MTT staining overlay method showed that the compound that had antibacterial activity was terpenoid in Macaranga conifora.
Kata Kunci : antibakteri, KLT-bioautografi, tumbuhan kawasan Borneo, Pseudomonas aeruginosa, antibacterial, TLC-bioautography, Borneo plants, Pseudomonas aeruginosa