PENGARUH PENAMBAHAN TAMANU (CALOPHYLLUM INOPHYLLUM L.) CRUDE OIL SEBAGAI ADITIF PADA RANSUM LOW ENERGY TERHADAP KARAKTERISTIK FERMENTASI RUMEN SECARA IN VITRO

Bayu Aji Saputro, Ir. Dimas Hand Vidya Paradhipta, S.Pt., M.Sc., Ph.D., IPP

2025 | Skripsi | ILMU DAN INDUSTRI PETERNAKAN

Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan tamanu (Calophyllum inophyllum L.) crude oil atau minyak biji tamanu (MBT) pada ransum low energy terhadap kecernaan, karakteristik fermentasi rumen, dan emisi gas metana (CH4) secara in vitro. Substrat pakan yang digunakan berupa rumput gajah (Pennisetum purpureum) dan konsentrat komersial, perbandingan hijauan dan konsentrat adalah 7:3 berdasarkan bahan kering dengan kadar protein kasar sebesar 8,18?n total digestible nutrient sebesar 53,44%. Pada tahap preparasi, MBT dilarutkan dalam pelarut lemak berupa petroleum benzene. Perlakuan terdiri dari penambahan MBT pada level 0 ppm (T0) dan 10 ppm (T1) ke dalam ransum. Masing-masing perlakuan dilakukan tiga kali replikasi selama dua periode inkubasi. Pada setiap periode in vitro menggunakan blanko sebanyak 2 botol dan standar rumput pangola sebanyak 2 botol. Sampel diinkubasi dengan Metode Tiley and Terry selama 48 jam pada suhu 39°C dengan perlakuan penggojokan setiap 8 jam sekali. Hasil penelitian menunjukkan bahwa penambahan MBT tidak berpengaruh secara nyata terhadap kecernaan bahan kering dan bahan organik. Selain itu, terjadi penurunan nilai pH rumen pasca inkubasi (P=0,025; 7,22±0,006 vs. 7,13±0,04) tetapi tidak diikuti denganperubahan amonia, volatile fatty acid total, dan CH4 yang menunjukan penambahan MBT hanya berpengaruh terhadap perubahan nilai pH tanpa berpengaruh nyata terhadap karakteristik fermentasi lain di dalam rumen seperti kecernaan, amonia, volatile fatty acid total, dan produksi gas CH4.

Kata kunci : in vitro, MBT, metana, ransum low energy, rumen

This study aims to determine the effect of using tamanu (Calophyllum inophyllum L.) crude oil (MBT) in low energy rations on digestibility, rumen fermentation characteristics, and methane (CH4) emissions in vitro. The feed substrate used consisted of Pennisetum purpureum and commercial concentrate, with a forage-to-concentrate ratio of 7:3 based on dry matter, containing 8.18% crude protein and 53.44% total digestible nutrients. In the preparation stage, the tamanu crude oil (MBT) was dissolved in a fat solvent, petroleum benzene. The treatment consisted of adding MBT at levels of 0 ppm (T0) and 10 ppm (T1) into the feed. Each treatment was replicated three times over two incubation periods. In each in vitro period, 2 bottles of blanks and 2 bottles of pangola grass standard were used. Samples were incubated using the Tiley and Terry Method for 48 hours at 39°C with shaking treatment every 8 hours. The research results show that the addition of MBT does not have a significant effect on the digestibility of dry matter and organic matter. Additionally, there was a decrease in rumen pH post-incubation (P=0.025; 7.22±0.006 vs. 7.13±0.04) but no changes in ammonia, total volatile fatty acids, and CH4, indicating that the addition of MBT only affected the pH value without significantly impacting other fermentation characteristics in the rumen such as digestibility, ammonia, total volatile fatty acids, and CH4 gas production.

Keywords: in vitro, MBT, methane, low-energy diet, rumen

Kata Kunci : in vitro, MBT, metana, ransum low energy, rumen