Keragaman Genetik, Produksi Biomassa, dan Kandungan Nutrien Rumput Rhodes (Chloris gayana cv. Callide) dengan Perendaman Kolkisin pada Regrowth Kedua dan Ketiga

Amarta Prameswari Purwasiwi, Prof. Ir. Nafiatul Umami, S.Pt., MP., Ph.D., IPM., ASEAN Eng; Ir. Yogi Sidik Prasojo, S.Pt., M.Agr., Ph.D., IPP

2025 | Tesis | S2 Ilmu Peternakan

Penelitian ini bertujuan untuk mengetahui pengaruh lama perendaman biji rumput rhodes pada larutan kolkisin 0,1?n faktor regrowth terhadap keragaman genetik, produksi biomassa, dan kandungan nutrien. Penelitian ini menggunakan Rancangan Acak Lengkap pola faktorial 4x2. Faktor pertama yakni lama perendaman (0, 2, 4, dan 6 jam) dan faktor kedua yakni fase regrowth (Regrowth 2 dan 3). Setiap level perlakuan terdapat 10 ulangan sehingga terdapat 40 satuan percobaan. Parameter yang diamati meliputi produksi biomassa, kandungan nutrien (bahan kering, bahan organik, serat kasar, lemak kasar, protein kasar), ukuran stomata, kandungan klorofil serta keragaman pada genetik tanaman. Data penelitian dianalisis menggunakan Analysis of variance (ANOVA), stomata daun dianalisis secara deskriptif, dan hasil keragaman genetik dianalisis menggunakan NTSYS (Numerical Taxonomy and Multivariate System). Hasil penelitian menunjukkan bahwa perendaman kolkisin (2, 4, dan 6 jam) dan regrowth berpengaruh nyata (P<0>regrowth memberikan pengaruh tidak signifikan (P<0>regrowth meningkatkan produksi biomassa, kandungan nutrien, dan karakteristik sitologi pada rumput rhodes. Interaksi keduanya akan berpengaruh pada kandungan protein kasar.

This research aims to determine the effect of soaking rhodes grass seeds in 0,1% colchicine and different regrowth phase on genetic diversity, biomass production and nutrient content. This research used a completely randomized design with two factors. The first factor is soaking time (0, 2, 4 and 6 hours) and the second factor is regrowth phase. Each treatment level had 10 replications so there were 40 experimental units. The parameters observed include biomass production, nutrient content (dry matter, organic matter, crude fiber, ether extract, crude protein), stomata size, chlorophyll content and genetic diversity. Data result were analyzed using Analysis of variance (ANOVA), leaf stomata were analyzed descriptively, and genetic diversity results were analyzed using NTSYS (Numerical Taxonomy and Multivariate System). The results showed that soaked with colchicine 0,1% (2, 4 and 6 hours) and regrowth phase had a significant effect (P<0>Regrowth factor had no significant influence (P>0.05) on crude fiber content in plants. Two treatment factors had a significant interaction (P<0>colchicine 0.1?n induction genetic diversity in rhodes grass with different primers characterized by the presence of polymorphic bands. Soaking for 6 hours with colchicine 0.1%  increased the size of the leaf stomata. The conclusion of this research is that the difference in soaking time with colchicine 0.1% and regrowth factor affects at biomass production, nutrient content and cytological characteristics in rhodes grass. Interaction between soaking time and regrowth affect for crude protein content.

Kata Kunci : Chloris gayana cv. Callide, Colchicine, RAPD, Regrowth, Stomata