EFEKTIVITAS PENAMBAHAN PROBIOTIK Lactobacillus casei 5%, 10%, DAN 15% PADA Aloe vera 15% TERHADAP DAYA HAMBAT PERTUMBUHAN Fusobacterium nucleatum (Kajian in vitro)

Selda Nathania, drg. Vincensia Maria Karina, MDSc., Sp.Perio(K); drg. Kwartarini Murdiastuti Sp.Perio(K)., Ph.D

2025 | Skripsi | PENDIDIKAN DOKTER GIGI

    Fusobacterium nucleatum adalah bakteri periodontopatogen utama yang termasuk golongan orange complex. Akumulasi plak yang berisi bakteri periodontopatogen perlu dihilangkan dengan scaling and root planing (SRP). Efektivitas SRP berkurang saat bakteri telah menginvasi jaringan periodontal sehingga diperlukan agen antibakteri yaitu Aloe vera. Penambahan probiotik Lactobacillus casei diperlukan untuk menghambat rekolonisasi bakteri. Penelitian ini bertujuan untuk mengetahui perbedaan efektivitas penambahan probiotik Lactobacillus casei 5%, 10%, dan 15% pada Aloe vera 15% terhadap daya hambat pertumbuhan Fusobaterium nucleatum.

        Metode penelitian uji daya hambat Fusobacterium nucleatum adalah difusi cakram pada Mueller Hinton Agar. Terdapat tiga kelompok perlakuan yaitu kelompok perlakuan penambahan probiotik Lactobacillus casei 5% pada Aloe vera 15%, penambahan probiotik Lactobacillus casei 10% pada Aloe vera 15%, penambahan probiotik Lactobacillus casei 15% pada Aloe vera 15%, kontrol positif (klorheksidin glukonat 0,2%), dan baseline (Aloe vera 15%). Diameter zona hambat diukur menggunakan jangka sorong kemudian data dianalisis menggunakan metode One-way ANOVA dan Post hoc LSD.

        Hasil penelitian menunjukkan perbedaan yang signifikan (p<0>Lactobacillus casei yang ditambahkan pada Aloe vera 15%, semakin besar daya hambat yang dihasilkan. Ketiga kelompok perlakuan memiliki daya hambat lebih besar dibandingkan dengan baseline namun tidak lebih besar dibandingkan dengan kontrol positif. Kesimpulan penelitian ini adalah penambahan probiotik Lactobacillus casei konsentrasi 15% pada Aloe vera 15% paling efektif dibandingkan konsentrasi 10?n 5% terhadap daya hambat pertumbuhan Fusobacterium nucleatum.

     Fusobacterium nucleatum is a major periodontopathogenic bacteria that belongs to the orange complex group. The accumulation of plaque containing these bacteria needs to be removed through scaling and root planing (SRP). The effectiveness of SRP can decrease when bacteria have invaded the periodontal tissue, necessitating the use of an antibacterial agent such as Aloe vera. The addition of probiotic Lactobacillus casei is needed to inhibit bacterial recolonization. This study aims to determine the difference in the effectiveness of adding probiotic Lactobacillus casei 5%, 10%, and 15% to Aloe vera 15% on the growth inhibition of Fusobacterium nucleatum.

      The research method for the Fusobacterium nucleatum inhibition test was disc diffusion on Mueller Hinton Agar. There are three treatment groups: 5%, 10%, and 15% Lactobacillus casei added to 15% Aloe vera, a positive control (0.2% chlorhexidine gluconate), and a baseline (15% Aloe vera). The diameter of the inhibition zone was measured using a sliding caliper, then the data were analyzed using the One-way ANOVA and Post hoc LSD methods.

       The results showed a significant differences (p <0>Lactobacillus casei added to 15% Aloe vera, the greater the inhibition zone. The three treatment groups had greater inhibition zone compared to the baseline but not greater than the positive control. The conclusion of this study is that the addition of 15% Lactobacillus casei to 15% Aloe vera is most effective compared to 10% and 5% concentrations in inhibiting the growth of Fusobacterium nucleatum.

Kata Kunci : Fusobacterium nucleatum, Lactobacillus casei, Aloe vera, antibakteri, daya hambat