PENGARUH WAKTU EKUILIBRASI TERHADAP KUALITAS SPERMA AYAM KAMPUNG YANG DIBEKUKAN DENGAN DIMETHYL FORMALMIDE SEBAGAI KRIOPROTEKTAN
Neni Daryanti , Dr. Ir. Kustono, M.Sc.,; Widya Asmarawati, S. Pt., M. Sc.
2014 | Skripsi | S1 PETERNAKANPenelitian ini bertujuan untuk mengetahui pengaruh berbagai waktu ekuilibrasi terhadap kualitas sperma yang dibekukan dengan Dimethyl formalmide (DMF) sebagai krioprotektan. Sperma ditampung dari 4 ekor ayam kampung jantan yang berumur sekitar 1,5 tahun dengan metode Burrows and Quinn. Sperma hasil penampungan diperiksa kualitas makroskopis dan mikroskopisnya, kemudian ditambahkan pengencer buffer phosphate yang telah diberi agen krioprotektan (Dimethyl formalmide 7 %). Sperma hasil pengenceran diekuilibrasi pada suhu 4 oC selama 0, 30,dan 60 menit, kemudian dibekukan di dalam nitrogen cair. Data motilitas, viabilitas, dan abnormalitas sperma dianalisis menggunakan rancangan acak lengkap pola searah. Hasil menunjukkan bahwa waktu ekuilibrasi 0, 30, dan 60 menit tidak signifikan terhadap motilitas, viabilitas dan abnormalitas sperma. Rerata motilitas spermatozoa berturut-turut adalah 66,00±1,41%; 65,75±1,50%; dan 66,00±2,45%, sedangkan rerata viabilitas spermatozoa berturut-turut 67,25±0,96%; 66,25±1,26%; 66,50±1,91% dan rerata abnormalitas spermatozoa berturut-turut 12,50±3,51%; 10,50±2,65%; 10,13±2,53%. Hasil pemeriksaan motilitas spermatozoa setelah thawing berturut-turut adalah 24,50±1,00%; 25,50±1,91%; dan 28,00±2,45%, sedangkan rerata viabilitas spermatozoa setelah thawing berturut-turut 25,50±0,58%; 26,25±2,06%; 28,25±2,06%. dan rerata abnormalitas spermatozoa setelah thawing berturut-turut 11,75±3,30%; 9,63±1,11%; 9,50±2,30%. Kesimpulan, waktu ekuilibrasi terhadap sperma ayam kampung tidak berpengaruh terhadap kualitas sperma. (Kata kunci : Sperma ayam kampung, Waktu ekuilibrasi, dan Dimethyl Formamide
The objective of the research was to identify the effect of equilibration times on the quality of chicken’s semen frozen with dimethyl formalmide as cryoprotectant. The semen was collected from four native chickens aged 1.5 years by using Burrows and Quinn method. The macroscopic and microscopic qualities of the semen were examined. After being examined, the fresh semen were added with buffer phospate as a diluent which had been added with 7% dimethyl formalmide. Semen were equilibrated in temperature 4 oC for 0, 30, and 60 minutes, then be frozen in liquid nitrogen. The data of motility, viability, and abnormality of semen were analyzed using completely randomized design one-way anova. The result showed that the equilibration times for 0, 30, and 60 minutes had no significant effect on motility, viability, and abnormality of semen. The mean motility of spermatozoa were 66.00±1.41%; 65.75±1.50%; and 66.00±2.45% while the average viability of spermatozoa were 67.25 ± 0.96%; 66.25 ± 1.26%; 66.50 ± 1.91% respectively. The mean abnormality spermatozoa were 12.50 ± 3.51%; 10.50 ± 2.65%; 10.13 ± 2.53% respectively. The spermatozoa motility after thawing were 24.50 ± 1.00%; 25.50 ± 1.91%; and 28.00 ± 2.45%, while the average viability of spermatozoa after thawing were 25.50 ± 0.58%; 26.25 ± 2.06%; 28.25 ± 2.06% respectively. The mean abnormality spermatozoa after thawing were 11.75 ± 3.30%; 9.63 ± 1.11%; 9.50 ± 2.30% respectively. Finally, the equilibration times of native chicken semen had no effect on semen quality. (Key words: Semen of Native chicken, Equilibration times, Dimethyl
Kata Kunci : Sperma ayam kampung, Waktu ekuilibrasi, dan Dimethyl Formalmide
