Pengaruh Kepadatan Inokulum Lactobacillus sp. GMP1 terhadap Pertumbuhan Raoultella ornithinolytica TN5 dengan Metode Co-culture
IHSANI GUSTITIA PATRIA, Indun Dewi Puspita, S.P., M.Sc., Ph.D.
2024 | Skripsi | TEKNOLOGI HASIL PERIKANANPenelitian ini bertujuan untuk mengetahui pengaruh kepadatan Lactobacillus sp. GMP1 pada metode co-culture terhadap laju pertumbuhan dan persen reduksi Raoultella ornithinolytica TN5. Lactobacillus sp. GMP1 dan R. ornithinolytica TN5 ditumbuhkan secara bersama pada medium TFIB dengan perlakuan jumlah inokulum 10^4:10^6 CFU/mL, 10^6:10^6 CFU/mL, dan 10^8:10^6 CFU/mL yang diinkubasi pada suhu 37°C selama 12 jam. Pada pengujian dilakukan perhitungan jumlah bakteri dengan perhitungan TPC. Data jumlah bakteri ini digunakan dalam perhitungan persen reduksi bakteri. Persen reduksi terbesar didapatkan pada perlakuan tiga dengan jumlah inokulum Lactobacillus sp. GMP1 dan R. ornithinolytica TN5 10^8:10^6 CFU/mL pada medium EMBA sebesar 64,598%. Selanjutnya data pertumbuhan log CFU/mL dan waktu inkubasi diplot dalam program DMFit. Pada laju pertumbuhan didapatkan laju pertumbuhan tertinggi pada perlakuan satu dengan jumlah inokulum Lactobacillus sp. GMP1 dan R. ornithinolytica TN5 10^4:10^6 CFU/mL pada medium TSA sebesar 0,127, medium EMBA sebesar 0,087, dan medium MRSA sebesar 0,168. Kadar histamin yang terbentuk selama waktu inkubasi dianalisis menggunakan Kromatografi Lapis Tipis (KLT) dan program imageJ dengan kadar histamin tertinggi yang terbentuk pada perlakuan satu sebesar 801 ppm. Nilai pH pada masing-masing perlakuan dilakukan pengujian menggunakan pH meter dengan nilai pH tertinggi yang didapatkan pada perlakuan satu sebesar 5,3. Selanjutnya dilakukan observasi morfologi bakteri pada sampel kontrol BAL, kontrol BPH, dan perlakuan tiga menggunakan SEM dan didapatkan hasil adanya perubahan morfologi sel bakteri pada perlakuan tiga.
This study aims to determine the effect of Lactobacillus sp. GMP1 density in the co-culture method on the growth rate and percentage reduction of Raoultella ornithinolytica TN5. Lactobacillus sp. GMP1 and R. ornithinolytica TN5 were grown together on TFIB medium with inoculum treatments of 10^4:10^6 CFU/mL, 10^6:10^6 CFU/mL, and 10^8:10^6 CFU/mL which were incubated at 37°C for 12 hours. In the test, the number of bacteria was calculated using the TPC calculation. This bacterial count data is used in calculating the percentage of bacterial reduction. The largest percentage reduction was obtained in treatment three with the amount of Lactobacillus sp. GMP1 and R. ornithinolytica TN5 inoculum 10^8:10^6 CFU/mL in EMBA medium was 64,598%. Futhermore, the log CFU/mL growth data and incubation time were plotted in the DMFit program. In terms of growth rate, the highest growth rate was obtained in treatment one with the amount of Lactobacillus sp. GMP1 and R. ornithinolytica TN5 inoculum 10^4:10^6 CFU/mL in TSA medium was 0,127, EMBA medium was 0,087, and MRSA medium was 0,168. The levels of histamine formed during the incubation time were analyzed using Thin Layer Chromatography (TLC) and the imageJ program with the highest histamine levels formed in treatment one of 801 ppm. The pH level in each treatment was tested using a pH meter with the highest pH level obtained in treatment one of 5,3. Futhermore, observations of bacterial morphology were carried out on BAL control, BPH control, and treatment three using SEM and the results obtained were changes in bacterial cell morphology in treatment three.
Kata Kunci : co-culture, Lactobacillus sp. GMP1, laju pertumbuhan, persen reduksi, Raoultella ornithinolytica TN5