Pengaruh Paparan Eco-enzyme Kulit Jeruk Pacitan (Citrus x aurantium L.) dan Serai (Cymbopogon citratus) Terhadap Destruksi Biofilm Bakteri Streptococcus sanguinis ATCC 10556 In Vitro
SAVIRA VRISCHA AMALIA, Dr. drg. Alma Linggar Jonarta, M.Kes.; Prof. Dr. drg. Juni Handajani, M.Kes., Ph.D.
2024 | Skripsi | ILMU KEPERAWATAN GIGI
Karies
dan penyakit periodontal bermula dari biofilm yang diinisiasi oleh bakteri
pionir salah satunya bakteri S. sanguinis.
Eco-enzyme
kulit jeruk Pacitan (Citrus x aurantium L.) dan serai (Cymbopogon
citratus) mengandung flavonoid, alkaloid, tanin, minyak
atsiri, senyawa fenolik, dan saponin yang mampu mengganggu proses quorum
sensing dan merusak matriks biofilm. Penelitian ini bertujuan untuk
mengetahui pengaruh eco-enzyme kulit jeruk Pacitan dan serai terhadap
destruksi biofilm bakteri S. sanguinis ATCC 10556 in vitro
Biofilm bakteri S. sanguinis
dibuat dengan cara mencampurkan 40 µl media BHI dengan 10 µl suspensi bakteri
konsentrasi 1,5 x 108 CFU/mL ke dalam microplate 96 well,
kemudian diinkubasi selama 24 jam pada suhu 37°C. Biofilm yang terbentuk
dipapar dengan 50 µl eco-enzyme dengan konsentrasi 40%, 20%, dan 10%, 50
µl chlorhexidine gluconate 0,2% sebagai kontrol positif, serta 50 µl PBS
sebagai kontrol negatif. Microplate kemudian diinkubasi kembali selama
24 jam pada suhu 37°C, selanjutnya diwarnai dengan kristal violet 0,1%. Pembacaan nilai optical density
menggunakan spektrofotometer dengan 450 nm. Selanjutnya data dianalisis
dengan One-Way ANOVA dan Post-Hoc LSD.
Hasil uji One-Way ANOVA
menunjukkan adanya perbedaan signifikan antar kelompok perlakuan.
Hasil uji Post-Hoc LSD menunjukkan terdapat perbedaan signifikan antara eco-enzyme
konsentrasi 40%, 20%, 10%, dan chlorhexidine
gluconate 0,1%. Kesimpulan dari
penelitian ini, eco-enzyme 40% memiliki kemampuan terbesar dibandingkan konsentrasi lainnya
dalam mendestruksi biofilm bakteri S. sanguinis, namun masih dibawah
kemampuan chlorhexidine gluconate 0,1%.
Caries and periodontal disease caused
by biofilms initiated by pioneer bacteria, such as S. sanguinis.
Eco-enzyme of Pacitan orange peels (Citrus x aurantium L.) and
lemongrass (Cymbopogon citratus) contains flavonoids, alkaloids,
tannins, essential oils, phenolic compounds, and saponins that interfere with
the quorum sensing process and damage the biofilm matrix. This study aimed to
determine the effect of eco-enzyme of Pacitan orange peels and lemongrass on
the destruction of S. sanguinis ATCC 10556 bacterial biofilm in vitro.
Streptococcus sanguinis bacterial biofilm was made by mixing 40 µl of BHI media with 10 µl of bacterial suspension with a concentration of 1.5 x 108 CFU/mL into a 96-well microplate, then incubated for 24 hours at 37°C. The biofilm formed was exposed to 50 µl of eco-enzyme with concentrations of 40%, 20%, and 10%, 50 µl of 0.2% chlorhexidine gluconate as a positive control, and 50 µl of PBS as a negative control. The microplate was then incubated again for 24 hours at 37°C, then stained with 0.1% crystal violet. Optical density values were read using a spectrophotometer 450 nm. Furthermore, the data were analyzed using One-Way ANOVA and Post-Hoc LSD.
The results of the One-Way ANOVA test showed significant differences between treatment groups. The results of the Post-Hoc LSD test showed significant differences between eco-enzyme concentrations of 40%, 20%, 10%, and chlorhexidine gluconate 0.1%. The conclusion of this study, eco-enzyme 40% has the greatest ability compared to other concentrations in destroying S. sanguinis bacterial biofilms, but is still below the ability of chlorhexidine gluconate 0.1%.
Kata Kunci : Eco-enzyme, destruksi biofilm, Streptococcus sanguinis
