PENGARUH PENAMBAHAN SERASAH DAUN JATI (Tectona grandis Linn.) TERHADAP KECERNAAN PAKAN DALAM RUSITEC

Utami Dwi Oktaviany, Dr. Ir. Supadmo. MS.; Ir. Chusnul Hanim, M.Si.

2013 | Skripsi | S1 PETERNAKAN

PENGARUH PENAMBAHAN SERASAH DAUN JATI (Tectona grandis Linn.) TERHADAP KECERNAAN PAKAN DALAM RUSITEC Utami Dwi Oktaviany 05/190913/EPT/00167 INTISARI Penelitian ini bertujuan untuk mengetahui pengaruh pemberian penambahan serasah daun jati terhadap kecernaan pakan in vitro Rumen Simulation Technique (RUSITEC). Perlakuan menggunakan penambahan serasah daun jati setara dengan level tanin 0, 0,1, 0,2, dan 0,3mg/ml medium. Pakan terdiri dari hijauan 60% dan konsentrat 40%. Dilakukan tiga kali pengulangan setiap perlakuan dengan masa inkubasi tiap ulangan selama 10 hari dengan RUSITEC. Sampel hasil in vitro dikeringkan pada suhu 55º C, kemudian dilakukan analisis proksimat untuk menghitung kecernaan bahan kering (KcBK), kecernaan bahan organik (KcBO), kecernaan serat kasar (KcSK), kecernaan protein kasar (KcPK) dan effluent di analisis aktivitas enzim CMC-ase. Data yang diperoleh kemudian dianalisis variansi pola searah kemudian dilanjutkan dengan uji Duncan’s multiple range test (DMRT). Penambahan level tanin dari serasah daun jati sampai 0,3mg/ml medium menurunkan KcPK dan KcBO (P<0,05), tetapi tidak berpengaruh tehadap KcBK, KcSK, dan aktivitas enzim CMCase. Hasil penelitian menunjukkan bahwa KcPK pada penambahan level tanin dari serasah daun jati sebesar 0, 0,1, 0,2, dan 0,3mg/ml medium berturut-turut 43,63%, 44,58%, 42,56%, 37,76% dan KcBO berturut-turut 22,91%, 23,08%, 23,38%, dan 19,87%. Dari hasil penelitian disimpulkan bahwa penambahan level 0,3mg/ml tanin serasah daun jati mampu memproteksi protein pakan dari degradasi nutrien pakan oleh mikrobia rumen tanpa berpengaruh negatif terhadap kecernaan pakan yang lainnya dan aktivitas enzim CMC-ase. Kata kunci: Serasah daun jati, RUSITEC, Kecernaan pakan, Aktivitas enzim CMC-ase.

THE EFFECT OF ADDITION OF TEAK LEAF (Tectona grandis Linn.) ON FEED DIGESTIBILITY IN RUSITEC Utami Dwi Oktaviany 05/190913/EPT/00167 ABSTRACT This study was aimed to know the effect of addition of teak leaf on feed digestibility using in vitro Rumen Simulation Technique (RUSITEC) method. The addition of teak leaf were 0, 0.1, 0.2 and 0.3 mg/ml of medium triplicate every in treatment. Sample of feed consisted of 60% king grass and 40% cattle consentrat. Incubation was carried out for 10 days in RUSITEC. At the end of fermentation undigested feed were dried at 55º C followed by analysed of chemical composition for meansuring dry matter digestibility (DMD), organic matter digestibility (OMD), crude fibre digestibility (CFD) and crude protein digestibility (CPD) while the medium of in vitro was analysed for CMC-ase activity. The data was analyzed variance using one way design and continued by Duncan’s new multiple range test (DMRT). Level tannin from teak leaf up to 0.3 mg/ml decreased CPD and OMD (P<0.05), but did not affect the DMD, CFD, and CMC-ase activities. The result showed that CPD with addition tannin at level 0, 0.1, 0.2 and 0.3 mg/ml of medium were 43.63%, 44.58%, 42.56%, 37.76% and OMD were 22.91%, 23.08%, 23.38% dan 19.87%, respectively. It could be concluded that the addition of teak leaf as a source of tannin by 0.3 mg/ml could protect feed protein from degradation by rumen microbia without negative effect on another nutritions digestibility and CMC-ase activity. Keyword: Teak leaf, RUSITEC, Feed digestibility and CMC-ase activity.

Kata Kunci : Serasah daun jati, RUSITEC, Kecernaan pakan, Aktivitas enzim CMC-ase