KAJIAN LINTAS SEKSIONAL RESISTANSI Salmonella spp. DAN Staphylococcus aureus TERHADAP STREPTOMISIN PADAANJING DI SHELTER KABUPATEN SLEMAN
AYU CAHYANEGORO, Dr. drh. Widagdo Sri Nugroho, M.P.
2024 | Skripsi | KEDOKTERAN HEWAN
Penanganan penyakit pada anjing dengan menggunakan antibiotik yang tidak rasional berpotensi mengakibatkan terjadinya resistansi antibiotik pada bakteri. Penelitian bakteri-bakteri resistan pada anjing perlu dilakukan. Penelitian ini bertujuan untuk mengetahui tingkat resistansi Salmonella spp. dan Staphylococcus aureus terhadap Streptomisin di 3 shelter anjing yang terdapat di Sleman, Daerah Istimewa Yogyakarta.
Penelitian ini menggunakan kajian lintas seksional dengan jumlah sampel sebanyak 147 ekor anjing yang diambil dengan random sederhana. Spesimen swab rektum dari setiap anjing dimasukkan ke dalam tabung berisi Buffered Peptone Water (BPW). Isolasi dan identifikasi Salmonella spp. dilakukan dengan menggunakan Xylose Lysine Deoxycholate (XLD), Triple Sugar Iron Agar (TSIA), dan Lysine Iron Agar (LIA). Isolasi dan identifikasi Staphylococcus aureus menggunakan Mannitol Salt Agar (MSA), Uji Katalase, Uji Koagulase, Uji Voges Proskauer (VP), dan Uji Gula. Pewarnaan Gram juga dilakukan untuk semua isolat. Uji sensitivitas bakteri terhadap streptomisin dilakukan dengan menggunakan Mueller-Hinton Agar (MHA) dengan dengan metode Kirby-Bauer. Data dianalisis secara deskriptif, univariat dan bivariat.
Hasil penelitian ini memperoleh 2 isolat Salmonella spp. dan 28 isolat Staphylococcus aureus dari 147 sampel. Sebanyak 1 isolat Salmonella spp. dan 1 isolat Staphylococcus aureus resisten terhadap streptomisin. Faktor risiko yang diperhatikan adalah asal hewan, pemberian antibiotik, dan gejala yang ditunjukkan selama 6 bulan terakhir. 50% Salmonella spp. dan 3,58% Staphylococcus aureus resistan terhadap streptomisin. Faktor risiko tidak menunjukkan adanya asosiasi antara resistansi Salmonella spp. dan Staphylococcus aureus terhadap streptomisin.
Disease management in dogs using irrational antibiotics has the potential to result in antibiotic resistance in bacteria. Research on resistant bacteria in dogs needs to be done. This study aims to determine the level of resistance of Salmonella spp. and Staphylococcus aureus to Streptomycin in 3 dog shelters located in Sleman, Yogyakarta Special Region.
This study used a cross-sectional study with a total sample size of 147 dogs taken by simple random. Rectal swab specimens from each dog were placed into tubes containing Buffered Peptone Water (BPW). Isolation and identification of Salmonella spp. was performed using Xylose Lysine Deoxycholate (XLD), Triple Sugar Iron Agar (TSIA), and Lysine Iron Agar (LIA). Isolation and identification of Staphylococcus aureus using Mannitol Salt Agar (MSA), Catalase Test, Coagulase Test, Voges Proskauer (VP) Test, and Sugar Test. Gram staining was also performed for all isolates. Bacterial sensitivity test to streptomycin was performed using Mueller-Hinton Agar (MHA) with Kirby-Bauer method. Data were analyzed descriptively, univariate and bivariate.
The results of this study obtained 2 isolates of Salmonella spp. and 28 isolates of Staphylococcus aureus from 147 samples. A total of 1 Salmonella spp. isolate and 1 Staphylococcus aureus isolate were resistant to streptomycin. Risk factors considered were animal origin, antibiotic administration, and symptoms exhibited during the last 6 months. 50% of Salmonella spp. and 3.58% of Staphylococcus aureus were resistant to streptomycin. Risk factors showed no association between the resistance of Salmonella spp. and Staphylococcus aureus to streptomycin.
Kata Kunci : anjing, Salmonella spp., shelter, Staphylococcus aureus, streptomisin, resistansi