Suplementasi Cairan Folikel Dalam Media Maturasi In Vitro Oosit Kambing Bligon

GRACIA DEFINNA KATILI, Prof. Ir. Diah Tri Widayati, M.P., Ph.D., IPM

2024 | Skripsi | ILMU DAN INDUSTRI PETERNAKAN

Penelitian ini dilakukan untuk mengetahui pengaruh suplementasi cairan folikel ovarium kambing pada medium maturasi oosit in vitro terhadap maturasi oosit kambing Bligon. Penelitian ini dilakukan di Laboratorium Fisiologi dan Reproduksi Ternak Fakultas Peternakan Universitas Gadjah Mada Yogyakarta dengan menggunakan ovarium kambing Bligon yang diperoleh dari rumah potong hewang (RPH) sekitar Yogyakarta. Ovarium dibawa dari RPH menggunakan larutan NaCl fisiologis bersuhu 31-34?. Koleksi oosit dilakukan melalui aspirasi menggunakan syringe yang diisi dengan flushing medium. Oosit dengan grade  A dan B  dicuci 3 kali dengan flushing medium dan di distribusikan ke dalam 50 mikroliter medium maturasi yang berbeda, yaitu tissue culture medium tanpa suplementasi cairan folikel, tissue culture medium + 10?iran folikel (CF), tissue culture medium + 20?iran folikel. Selanjutnya ditutup dengan minyak mineral dan diinkubasi dalam inkubator pada suhu 38? kelembaban udara 95?n kadar 5% CO2 selama 24 jam. Variabel yang diamati adalah angka dan kualitas maturasi oosit. Maturasi oosit diukur berdasarkan ekspansi sel kumulus. Data angka maturasi dianalisis statistik dengan menggunakan analisis variansi pola searah menggunakan SPSS 25, kualitas maturasi oosit dianalisis secara kualitatif. Hasil penelitian menunjukkan bahwa penambahan cairan folikel berpengaruh nyata pada maturasi oosit in vitro (P<0>mature  oosit kambing Bligon lebih baik dibandingkan dengan culture medium dengan 10% suplementasi maupun tanpa suplementasi.

This research was conducted to determine the effect of ovarian follicular fluid supplementation on in vitro oocyte maturation medium on the maturation of Bligon goat oocytes. This research was conducted at the Physiology and Animal Reproduction Laboratory, Faculty of Animal Husbandry, Universitas Gadjah Mada, Yogyakarta, using Bligon goat ovaries obtained from slaughterhouses around Yogyakarta. The ovaries were transported from the slaughterhouse using physiological NaCl solution at a temperature of 31-34°C. Oocyte collection was performed by aspiration using a syringe filled with flushing medium. Grade A and B oocytes were washed three times with flushing medium and distributed into 50 microliter of different maturation media, namely tissue culture medium without follicular fluid supplementation, tissue culture medium + 10% follicular fluid (FF), tissue culture medium + 20% follicular fluid. Next, they were covered with mineral oil and incubated in an incubator at a temperature of 38°C, 95% air humidity, and 5% CO2 concentration for 24 hours. The variables observed were the number and quality of oocyte maturation. Oocyte maturation was measured based on cumulus cell expansion. Maturation data were analyzed statistically using a one-way analysis of variance with SPSS 25, while oocyte maturation quality was analyzed qualitatively. The results showed that the addition of follicular fluid significantly affected in vitro oocyte maturation (P<0>

Kata Kunci : Cairan folikel, maturasi oosit in vitro, kambing Bligon, suplementasi