Pengaruh Konsentrasi Medium Putih Telur yang Berbeda pada Pemisahan Spermatozoa Terhadap Kualitas dan Proporsi Spermatozoa X dan Y Kambing Saanen

RENDI MAULANA AKSAN, Prof. Dr. Ir. Sigit Bintara, M.Si., IPU., ASEAN Eng.

2024 | Skripsi | ILMU DAN INDUSTRI PETERNAKAN

Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi medium putih telur yang berbeda pada pemisahan spermatozoa terhadap kualitas dan proporsi spermatozoa X dan Y kambing Saanen pada fraksi atas dan fraksi bawah setiap medium yang berbeda: 1) P0 = sperma tanpa konsentrasi putih telur (kontrol); 2) P1 = konsentrasi 10% + 30%; 3) P2 = konsentrasi 15% + 45%; 4) P3 = konsentrasi 25% + 75%. Semen segar kambing Saanen jantan berumur 3 tahun dikoleksi menggunakan vagina buatan, dilakukan penilaian secara makroskopis (volume, pH, warna, bau, konsistensi), serta mikroskopis (motilitas, viabilitas, abnormalitas). Data rerata kualitas berupa motilitas, viabilitas, dan abnormalitas pasca pemisahan; dan rerata proporsi spermatozoa X dan Y berdasarkan panjang ×  lebar kepala dianalisis variansi data Rancangan Acak Lengkap (RAL) Pola Searah, kemudian dilanjutkan uji Duncan’s Multiple Range Test (DMRT) untuk data perbedaan yang nyata. Hasil analisis menunjukkan pemisahan dengan konsentrasi medium putih telur berpengaruh nyata meningkatkan proporsi spermatozoa X lapisan atas dan meningkatkan proporsi spermatozoa Y lapisan bawah (P<0>

This study aims to determine the impact of varying concentrations of egg white medium on the separation of spermatozoa in Saanen goats. The study specifically focuses on assessing the quality and quantity of X and Y spermatozoa in both the upper and lower fractions of each medium. 1) P0 represents sperm without egg white concentration, which serves as the control group. 2) P1 represents a concentration of 10% egg white combined with 30% other substances. 3) P2 represents a concentration of 15% egg white combined with 45% other substances. 4) P3 represents a concentration of 25% egg white combined with 75% other substances. The semen from 3-year-old male Saanen goats was obtained through the use of an artificial vagina and examined both macroscopically (for volume, pH, colour, odour, and consistency) and microscopically (for motility, viability, and abnormalities). The data collected after separation was analysed using a Completely Randomised Design (CRD) and Duncan's Multiple Range Test (DMRT) to determine significant changes in motility, viability, abnormalities, and the fraction of X and Y spermatozoa depending on head length and width. The findings demonstrated that varying concentrations of egg white medium had a substantial impact on the proportion of X spermatozoa in the upper layer and Y spermatozoa in the lower layer (P<0>

Kata Kunci : kambing Saanen, medium putih telur, pemisahan spermatozoa X dan Y