PENGARUH PEMBERIAN BATANG BROKOLI (Brassica oleracea var. Italica) TERHADAP BOBOT BADAN, BOBOT TESTIS, DAN GONADO SOMATIK INDEKS TIKUS PUTIH (Rattus norvegicus) SPRAGUE-DAWLEY
PRIMA MEILANISA ISHMAH, drh. Yuda Heru Fibrianto, M.P., Ph.D.
2024 | Skripsi | KEDOKTERAN HEWAN
Penelitian
ini bertujuan mengkaji pengaruh pemberian batang brokoli terhadap bobot badan,
bobot testis, dan gonado somatik indeks (GSI) tikus putih jantan galur Sprague-Dawley.
Pada penelitian ini menggunakan hewan percobaan tikus strain Sprague-Dawley jantan, umur 3 bulan, dan
mempunyai bobot badan 175 – 183 gram, sebanyak 25 ekor. Hewan percobaan dibagi menjadi 5
kelompok perlakuan yaitu kelompok kontrol
negatif (P1) dengan perlakuan aquades, kelompok kontrol
positif (P2) dengan perlakuan Letrozole, dan kelompok P3, P4, dan P5 masing-masing diberikan perlakuan brokoli dengan
dosis 200, 400, dan 800 mg/200 gr BB/hari. Pemberian intervensi secara per oral selama 21 hari, pengukuran bobot badan dilakukan setiap 7 hari, pada
hari ke-21 semua tikus dinekropsi
dan dilakukan pengukuran bobot testis. Hasil penelitian menunjukkan bahwa rerata bobot badan
P1 207,00
± 2,236 gram, P2 207,80 ± 4,494 gram, P3 205,20 ± 2,588 gram, P4 206,60 ±
2,408 gram, dan P5 206,20 ± 3,194. Bobot
testis P1 sebesar 6,36 ± 1,21 gram, P2 5,24 ± 0,86 gram, P3 5,05 ± 0,51
gram, P4 5,36 ± 1,30 gram, dan P5 5,11 ± 0,61 gram. Nilai GSI diperoleh dari hasil perbandingan antara
bobot testis dan bobot badan lalu dikalikan 100?ngan nilai P1 sebesar 3,07 ±
0,60 %, P2 2,53 ± 0,41 %, P3 2,46 ± 0,25 %, P4 2,59 ± 0,63 %, dan P5 2,48 ±
0,29 %. Hasil analisis statistik menunjukkan bahwa pemberian batang brokoli tidak berpengaruh signifikan (P>0,05) terhadap bobot badan, bobot testis dan GSI. Berdasarkan hasil penelitian dapat disimpulkan bahwa
pemberian batang brokoli selama 21 hari tidak berpengaruh terhadap bobot badan,
bobot testis dan GSI
Kata kunci: Brokoli, tikus, bobot
badan, bobot testis, GSI
This
study aimed to examine the effect of giving broccoli stalk on the body weight,
testes weight, and gonado-somatic-index (GSI) of male Sprague-Dawley rats. In
this study using experimental animals male Sprague-Dawley strain rats, aged 3
months, and have a body weight of 175-183 grams, as many as 25 heads.
Experimental animals were divided into 5 treatment groups, namely the negative
control group (P1) with aquades treatment, the positive control group (P2) with
Letrozole treatment, and the P3, P4, and P5 groups were each given broccoli
treatment at doses of 200, 400, and 800 mg/200 g BW/day per oral intervention
for 21 days. The body weight measurement was carried out every 7 days, on the 21st
day all rats were necropsy and testicular weight measurements were taken. The
results showed that the average body weight of P1 207.00 ± 2.236 grams, P2
207.80 ± 4.494 grams, P3 205.20 ± 2.588 grams, P4 206.60 ± 2.408 grams, and P5
206.20 ± 3.194. Testicular weights of P1 were 6.36 ± 1.21 grams, P2 5.24 ± 0.86
grams, P3 5.05 ± 0.51 grams, P4 5.36 ± 1.30 grams, and P5 5.11 ± 0.61 grams. The
GSI value is obtained from the comparison between testicular weight and body
weight and then multiplied by 100% with a value of P1 was 3.07 ± 0.60 %, P2
2.53 ± 0.41 %, P3 2.46 ± 0.25 %, P4 2.59 ± 0.63 %, and P5 2.48 ± 0.29 %. The
results of statistical analysis showed that broccoli administration had no
significant effect (P>0.05) on body weight, testicular weight, and GSI.
Based on the results of the study, it can be concluded that giving broccoli
stalk for 21 days has no effect on body weight, testicular weight, and GSI.
Keywords:
Broccoli, rat, body weight, testicular weight, GSI
Kata Kunci : Kata kunci: Brokoli, tikus, bobot badan, bobot testis, GSI
