Kajian Lintas Seksional Resistansi Salmonella spp. dan Staphylococcus aureus terhadap Tetrasiklin pada Anjing di Shelter Kabupaten Sleman
SHAFIRA AULIA PUTRI, Dr. drh. Widagdo Sri Nugroho, M.P.
2024 | Skripsi | KEDOKTERAN HEWAN
Antimicrobial resistance (AMR) is an increasing global threat to public and animal health. This problem occurs in many pathogens and commensals originating from dogs, including Salmonella spp. and Staphylococcus aureus. Tetracycline is a broad-spectrum antibiotic that is one of the choices used in treatment. This study aims to determine the level of resistance of Staphylococcus aureus and Salmonella spp. to tetracycline and determine the risk factors for the presence of Salmonella spp. and Staphylococcus sp. in dogs.
A total of 147 rectal swab specimens were collected from dogs from three different shelters in Sleman Regency, the Special Region of Yogyakarta (DIY) with a cross-sectional study approach. The sampling technique used was simple random. Isolation and identification of Salmonella spp. using Xylose Lysine Deoxycholate (XLD), Triple Sugar Iron Agar (TSIA), and Lysine Iron Agar (LIA), and Gram stain media. Isolation and identification of Staphylococcus aureus using Mannitol Salt Agar (MSA) media, Gram stain, catalase test, coagulase test, sugar (mannitol) test, and Voges-Proskauer (VP) test. Isolates confirmed as Salmonella spp. and Staphylococcus aureus continued with resistance testing against tetracycline using the Kirby Bauer method. Data analysis was descriptive, univariate, and bivariate.
The types of dogs found in the shelter consisted of 96 domestic dogs, 25 pedigree dogs, and 26 mixed dogs with 89 males and 58 females. The health status of dogs is known to be 141 healthy and 6 diarrhea. The results of isolation and identification obtained two isolates (1.4%) Salmonella spp. and 28 isolates (19%) Staphylococcus aureus infected dogs. One Salmonella spp. isolate (50%) and 10 Staphylococcus aureus isolates (35.7%) were identified as tetracycline-resistant. Risk factor analysis of sex, breed, age, history of diarrhea, antibiotic treatment, feeding and drinking were not associated with the presence of Salmonella spp. and Staphylococcus sp. and antibiotic resistance. The resistance rate of Salmonella spp. and Staphylococcus aureus to tetracycline was 50% and 35.7%. No risk factors were found that specifically triggered the presence of Salmonella spp. and Staphylococcus aureus and their resistance to tetracycline.
Kata Kunci : Anjing, Shelter, Salmonella spp., Staphylococcus aureus, Resistansi, Tetrasiklin