COX-2 EXPRESSION AND NEUTROPHIL NUMBER AFTER PULPOTOMY WITH COMPOSITE GELATIN - CHITOSAN - TEOS-Ca(OH)2 OR Ca(OH)2

Ghadah abdulrahman alqatta, Prof. Dr. drg. Juni Handajani, M.Kes; Dr. drg. Retno Ardhani, M.Sc

2024 | Tesis | S2 Kedokteran Gigi

<!--[if gte mso 9]><xml> Normal 0 false false false EN-ID X-NONE AR-SA </xml><![endif]--><!--[if gte mso 9]><xml> </xml><![endif]--><!--[if gte mso 10]> <style> /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-pagination:none; text-autospace:none; font-size:11.0pt; font-family:"Calibri",sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:Arial; mso-bidi-theme-font:minor-bidi; mso-ansi-language:EN-US; mso-fareast-language:EN-US;} </style> <![endif]-->

Introduction: Calcium hydroxide (Ca(OH)2) is the material of choice for vital pulp therapies. However, Ca(OH)2 has drawbacks because of the toxicity, poor sealing and tunnel defect formation tendency. Alternative materials were developed to provide a more biocompatible with better dentin formation ability agents. In this study, we evaluate the effect of (G), (CH), (TEOS) and Ca(OH)2 composite, namely G/CH/TEOS/Ca(OH)2 compare to Ca(OH)2 on the inflammation of the dental pulp. Materials and methods: In this study, a total of 16 Wistar rat models of an acute pulp injury was prepared and divided into 2 group, namely treatment and control,

8 each. The treatment group were applied pulp capping material using G/CH/TEOS/Ca(OH)2 and the control used Ca(OH)2 . On the 1^st and 3^rd days, rats were sacrificed. Tissue from 4 rats each group processed for histological preparation. Samples were stained using Hematoxylin-Eosin to examine neutrophils number and immunohistochemistry using anti-COX-2 antibody to identify COX-2 expression. Results: The results showed that neutrophils number and COX-2 expression were decreasing significantly in the treatment group compared to the control group by Kruskal Wallis and ANOVA (p<0 xss=removed>G/CH/TEOS/Ca(OH)2 affects the number of neutrophils and COX-2 expression. The post hoc result showed that the number of neutrophils and COX-2 expression were not significantly lower in the treatment group compared to control group each observation day by Mann-Whitney and Dunnett T-3 test. It is indicating that G/CH/TEOS/Ca(OH)₂ and Ca(OH)₂ have same effect on neutrophils number and COX-2 expression. The decrease in COX-2 expression and in neutrophils number in dental pulps after pulpotomy with G/CH/TEOS/Ca(OH)₂ compared to Ca(OH)2 this findings suggest that the composite material provokes a lower inflammatory processes in the pulp tissue by decreasing C. Conclusion: Gelatin Chitosan TEOS Ca(OH)₂ composite have an effect on decreasing the neutrophils number and COX-2 expression in the dental pulp of Wistar rats that experience inflammation.

<!--[if gte mso 9]><xml> Normal 0 false false false EN-ID X-NONE AR-SA </xml><![endif]--><!--[if gte mso 9]><xml> </xml><![endif]--><!--[if gte mso 10]> <style> /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-pagination:none; text-autospace:none; font-size:11.0pt; font-family:"Calibri",sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:Arial; mso-bidi-theme-font:minor-bidi; mso-ansi-language:EN-US; mso-fareast-language:EN-US;} </style> <![endif]-->

Introduction: Calcium hydroxide (Ca(OH)2) is the material of choice for vital pulp therapies. However, Ca(OH)2 has drawbacks because of the toxicity, poor sealing and tunnel defect formation tendency. Alternative materials were developed to provide a more biocompatible with better dentin formation ability agents. In this study, we evaluate the effect of (G), (CH), (TEOS) and Ca(OH)2 composite, namely G/CH/TEOS/Ca(OH)2 compare to Ca(OH)2 on the inflammation of the dental pulp. Materials and methods: In this study, a total of 16 Wistar rat models of an acute pulp injury was prepared and divided into 2 group, namely treatment and control,

8 each. The treatment group were applied pulp capping material using G/CH/TEOS/Ca(OH)2 and the control used Ca(OH)2 . On the 1^st and 3^rd days, rats were sacrificed. Tissue from 4 rats each group processed for histological preparation. Samples were stained using Hematoxylin-Eosin to examine neutrophils number and immunohistochemistry using anti-COX-2 antibody to identify COX-2 expression. Results: The results showed that neutrophils number and COX-2 expression were decreasing significantly in the treatment group compared to the control group by Kruskal Wallis and ANOVA (p<0 xss=removed>G/CH/TEOS/Ca(OH)2 affects the number of neutrophils and COX-2 expression. The post hoc result showed that the number of neutrophils and COX-2 expression were not significantly lower in the treatment group compared to control group each observation day by Mann-Whitney and Dunnett T-3 test. It is indicating that G/CH/TEOS/Ca(OH)₂ and Ca(OH)₂ have same effect on neutrophils number and COX-2 expression. The decrease in COX-2 expression and in neutrophils number in dental pulps after pulpotomy with G/CH/TEOS/Ca(OH)₂ compared to Ca(OH)2 this findings suggest that the composite material provokes a lower inflammatory processes in the pulp tissue by decreasing C. Conclusion: Gelatin Chitosan TEOS Ca(OH)₂ composite have an effect on decreasing the neutrophils number and COX-2 expression in the dental pulp of Wistar rats that experience inflammation.

Kata Kunci : Ca(OH)2 , Pulpotomy, Neutrophils number, COX-2 expression.