Perbedaan daya anti bakteri ChKM, Cresophene dan formula Rockle No.4 terhadap pertumbuhan Peptostreptococcus dan Bacteroides penyebab infeksi saluran akar

PRABOWO, Andy Shantyo, Prof.drg. H.R. Moendjaeni P., SpKG

2004 | Tesis | PPDGS I Ilmu Konservasi Gigi

Tujuan penelitian ini adalah untuk mengetahui perbedaan daya antibakteri antara ChKM, cresophene dan formula Rockle No. 4 dalam menghambat pertumbuhan bakteri Peptostreptococcus dan Bacteroides penyebab infeksi saluran akar yang diisolasi klinis secara in vitro. Uji bakteri metode difusi agar, digunakan untuk evaluasi kemampuan antiseptik intrakanal dalam menghambat pertumbuhan bakteri yang sering ditemukan pada gigi nekrosis pulpa. Tigapuluh (30) cawan Petri berisi 10 ml agar Brain Heart Infusion (B.H.I) dibagi 2 kelompok untuk masing-masing bakteri. Tiap cawan Petri dibuat 4 sumuran, 3 untuk antiseptik intrakanal dan 1 untuk kontrol negatif. Masing masing disinfektan intrakanal diteteskan 10 μl dalam setiap sumuran pada 5 cawan Petri kemudian semua sampel dieramkan selama 48 jam suhu 37oC dalam inkubator. Uji biakan agar gores digunakan untuk evaluasi efek bakterisid antiseptik intrakanal terhadap bakteri tersebut. Campuran antiseptik dan bakteri dimasukkan dalam 30 flask berisi agar B.H.I, dieramkan selama 48 jam suhu 370C. Pertumbuhan bakteri diukur dari ada tidaknya turbiditas atau kekeruhan media kultur. Hasil menunjukkan ChKM, cresophene dan formula Rockle No. 4 mempunyai daya antibakteri yang berbeda. Dalam biakan agar B.H.I, formula Rockle No. 4 menunjukkan zona hambatan bakteri yang terluas diikuti ChKM kemudian Cresophene. Hasil uji biakan agar gores menunjukkan daya anti bakteri ChKM, Cresophene dan Formula Rockle No. 4 terhadap Peptostreptococcus pada 15 sampel masing-masing adalah 93,3%, 80% dan 100%, sedangkan terhadap Bacteroides pada 15 sampel, semua antiseptik memiliki daya antibakteri 100%. Dari analisis statistik anova 2 ekor dan uji t serta Chi kuadrat ternyata tidak ada perbedaan bermakna daya antibakteri antara ChKM dan Cresophene (p>0,01) terhadap kedua jenis bakteri. Daya antibakteri formula Rockle No. 4 berbeda bermakna secara statistik dibanding ChKM dan Cresophene (p<0,01) terhadap kedua jenis bakteri anaerob penyebab infeksi saluran akar.

The purpose of this research was to know the difference of antibacterial activities of camphorated paramonochlorophenol (CMCP), cresophene and antiseptic Rockle’s No. 4 formula on the growth inhibitory of clinical isolated Peptostreptococcus and Bacteroides causing root canal infection in-vitro. In the laboratory experiment, the agar diffusion test was used to evaluate the inhibitory activity of the intracanal medicament against bacteria commonly found in teeth necrotic pulp. Thirty (30) Petri plates with 10 ml Brain Heart Infusion (B.H.I) agar devided into two equal group of each bacterial species. Each Petri plate consist of four wells, three for medicaments and one for negative control which were dropped with 10 ml medicament in 5 Petri plates. All samples incubated at 370C for 48 hours. Agar scratch-culture test was also used to evaluate bacterial effect of intracanal medicaments against those microorganism.The bacterial-medicament solutions were swabed on 30 flask of 10 ml B.H.I. agar and incubated at 370C for 48 hours. The microbial growth was evaluated by turbidity of the culture media. The results showed that all medicaments had difference antibacterial activities. Rockle’s No. 4 formula showed the largest zone of bacterial inhibition growth among all bacterial species tested in B.H.I. agar cultures. The agar scratch culture test results showed that antibacterial activity precentage of CMCP, Cresophene and antiseptic Rockle’s No. 4 formula were 93,3%, 80% and 100% against Peptostreptococcus meanwhile against Bacteroides, all medicaments had 100% result from 15 samples. From 2-tailed anova statistic analysis, t-test and Chi-square resulted that there were no significant difference on antimicrobial activity between CMCP and cresophene (p>0,01) to these bacterias. There were significant difference between antiseptic Rockle’s No. 4 formula to CMCP and cresophene (p<0,01) againt Peptostretococcus and Bacteroides causing root canal infection.

Kata Kunci : Gigi, Infeksi Saluran Akar, Anti Bakteri ChKM, Cresophene dan Formula Rockle No 4